System Biosciences
PB-EF1a-Oct4-Sox2-Klf4-Myc-IRES-GFP Human 4-in-1 iPSC Vector
- SKU:
- PB630A-1
- UPC:
- MPN:
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 10 ug
- Shipping Temperature:
- Blue Ice/ Dry Ice
- Qualifications:
- Requires signed license from commercial customers
Description
PB-EF1a-Oct4-Sox2-Klf4-Myc-IRES-GFP Human 4-in-1 iPSC Vector. Cat# PB630A. Supplier: SBI System Biosciences
- Make transgenic cell lines with a single transfection
- Integrate multiple PiggyBac Vectors in a single transfection
- Insert an expression cassette into human, mouse, and rat cells
- Deliver virtually any-sized DNA insert, from 10 – 100 kb
- Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or inducible promoters and include a variety of markers
Overview

- Make transgenic cell lines with a single transfection
- Integrate multiple PiggyBac Vectors in a single transfection
- Insert an expression cassette into human, mouse, and rat cells
- Deliver virtually any-sized DNA insert, from 10 – 100 kb
- Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or inducible promoters and include a variety of markers
- Determine the number of integration events with the PiggyBac qPCR Copy Number Kit (# PBC100A-1)
- Academic Customers—End-User License Information
- Commercial Customers—End-User License Information
How It Works
The PiggyBac Transposon System’s Cut-and-Paste Mechanism
The efficient PiggyBac Transposon System uses a cut-and-paste mechanism to transfer DNA from the PiggyBac Vector into the genome. If only temporary genomic integration is desired, the Excision-only PiggyBac Transposase can be transiently expressed for footprint-free removal of the insert, resulting in reconstitution of the original genome sequence.
Figure 1. The PiggyBac Transposon System’s cut-and-paste mechanism.
- The Super PiggyBac Transposase binds to specific inverted terminal repeats (ITRs) in the PiggyBac Cloning and Expression Vector and excises the ITRs and intervening DNA.
- The Super PiggyBac Transposase inserts the ITR-Expression Cassette-ITR segment into the genome at TTAA sites.
- The Excision-only Super PiggyBac Transposase can be used to remove the ITR-Expression Cassette-ITR segment from the genome, for footprint-free removal
Supporting Data
Easily reprogram human cells with the PiggyBac Transposon System
Figure 2. Easily reprogram human cells with the PiggyBac Transposon System. Human neonatal skin fibroblasts were co-transfected with PB-EF1α-Oct4-Sox2-Klf4-Myc-IRES-GFP Human 4-in-1 iPSC PiggyBac Vector (Cat.# PB630A-1) and the Super PiggyBac Transposase Expression Vector (Cat.# PB210PA-1). iPS cells were derived using morphological selection criteria. When cultured under standard human ES cell culture conditions, the morphology of the custom human iPS cells was identical to that of human ES cells. Additionally, the cells express the pluripotency markers Oct4, Nanog, SSEA3, TRA-1-60, and demonstrate strong endogenous alkaline phosphatase staining.
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