PB-SV40-Neo-EF1-GFP-CAGs-cMyc-Klf4-Oct4-Sox2 Mouse 4-in-1 iPSC Vector

Easily reprogram mouse cells with the PiggyBac Transposon System

Figure 2. Easily reprogram mouse cells with the PiggyBac Transposon System. (A) Gene expression of the PiggyBac Mouse 4-in-1 iPSC Vector was analyzed in 3T3 Cells transfected with either (- lane) the Super PiggyBac Transposase Expression Vector (Cat.# PB210PA-1) alone or (+ lane) the Super PiggyBac Transposase Expression Vector and the PiggyBac Mouse 4-in-1 iPSC Vector. Cells were harvested at two weeks post-transfection, and RNA was isolated and reverse transcribed (RT) into cDNA. Gene expression was evaluated via PCR using primers specific for the four factors and a Gapdh loading control. Only the cells co-transfected with the PiggyBac Mouse 4-in-1 iPSC Vector showed expression of the transcripts. (B). Cells from (A) were also imaged for GFP expression and stained with anti-Sox2 antibody (Texas Red, antibody in the + panel only). DAPI stain was used as a positive control to identify cell nuclei.