System Biosciences
pGF-CREB-mCMV-EF1-Puro (plasmid)
- SKU:
- TR202pa-p
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 10 ug
- Shipping Temperature:
- Blue Ice/ Dry Ice
Description
pGF-CREB-mCMV-EF1-Puro (plasmid). Cat# TR202pa-p. Supplier: SBI System Biosciences
Study oncogenic signaling by co-expressing dscGFP and luciferase in response to CREB activity at CREB transcriptional response elements
- Sort responsive cells with dscGFP
- Measure activity with luciferase
- Leverage SBI’s highly-regarded lentivectors
- Create stable signaling pathway reporter cell lines
- Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines
Products
Overview
Monitor oncogenic signaling in real time With SBI’s line of pGreenFire1 Pathway Reporters, you can monitor signal transduction in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built signal transduction pathway reporters come as ready-to-package lentivector plasmid*, ready-to-transduce pre-packaged lentivirus, and as a ready-to-study cell line. The pGF-CREB-mCMV-EF1α-Puro Lentivector co-expresses a destabilized copepod GFP (dscGFP; 2-hour half-life) and luciferase from CREB transcriptional response elements (TREs) paired with a minimal CMV promoter (mCMV).
The mCMV promoter alone delivers negligible expression, but when downstream of CREB TREs, drives expression of dscGFP and luciferase in response to CREB activity. The result is the ability to quantitatively measure CREB activity at CREB TREs by fluorescence and luciferase activity.
- Sort responsive cells with dscGFP
- Measure activity with luciferase
- Leverage SBI’s highly-regarded lentivectors
- Create stable signaling pathway reporter cell lines
- Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines
The pGF-CREB-mCMV-EF1α-Puro Lentivector is available as a lentivector, pre-packaged virus, and a stable cell line created from the pGF-CREB-mCMV-EF1α-Puro Lentivector transduced into 293 cells.
*Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.Supporting Data
See our transcriptional response element reporters in action
Monitor oncogenic pathway reporters
Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis
General pGreenFire data examples
Monitoring NF-κB transactivation
Related Products
pGF-CREB-mCMV-EF1-Puro (virus)
System Biosciences
zł3,888.42
pGF-CREB-mCMV-EF1-Puro (virus). Cat# TR202va-p. Supplier: SBI System Biosciences
Study oncogenic signaling by co-expressing dscGFP and luciferase in response to CREB activity at CREB transcriptional...
pGF-EGR1-mCMV-EF1-Puro (plasmid)
System Biosciences
zł3,888.42
pGF-EGR1-mCMV-EF1-Puro (plasmid). Cat# TR204pa-p. Supplier: SBI System Biosciences
Study oncogenic signaling by co-expressing dscGFP and luciferase in response to EGR1 activity at EGR1...
pGF-AP1-mCMV-EF1-Puro (plasmid)
System Biosciences
zł3,888.42
pGF-AP1-mCMV-EF1-Puro (plasmid). Cat# TR201pa-p. Supplier: SBI System Biosciences
Study oncogenic signaling by co-expressing dscGFP and luciferase in response to AP1 activity at AP1 transcriptional...
pGF-p53-mCMV-EF1-Puro (plasmid)
System Biosciences
zł3,888.42
pGF-p53-mCMV-EF1-Puro (plasmid). Cat# TR200pa-p. Supplier: SBI System Biosciences
Study oncogenic signaling by co-expressing dscGFP and luciferase in response to p53 activity at p53 transcriptional...
pGF-CREB-mCMV-EF1-Puro (293 stable cell line)
System Biosciences
zł16,987.22
pGF-CREB-mCMV-EF1-Puro (293 stable cell line). Cat# TR202a-p. Supplier: SBI System Biosciences
Study oncogenic signaling by co-expressing dscGFP and luciferase in response to CREB activity at CREB...