System Biosciences
pLL-EF1a-rFLuc-T2A-mRFP-mPGK-Puro [Lenti-Labeler™ plasmid]
- SKU:
- LL420PA-1
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 10 ug
- Shipping Temperature:
- Blue Ice/ Dry Ice
Description
pLL-EF1a-rFLuc-T2A-mRFP-mPGK-Puro [Lenti-LabelerTM plasmid]. Cat# LL420PA. Supplier: SBI System Biosciences
- Proven—leverages SBI’s third generation lentivector technology for optimal virus titers
- Validated—sequence-verified from LTR to LTR and expression-verified in HT-1080 cells
- Flexible—available in a range of fluorescent markers, selection markers, and promoters (Table 1)
Products
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Overview
![](https://cdn11.bigcommerce.com/s-i3n9sxgjum/product_images/uploaded_images/moved-6384c842568db8f81579e645b7f6bdcf.png)
- Proven—leverages SBI’s third generation lentivector technology for optimal virus titers
- Validated—sequence-verified from LTR to LTR and expression-verified in HT-1080 cells
- Flexible—available in a range of fluorescent markers, selection markers, and promoters (Table 1)
SBI offers Lenti-Labeler constructs with a range of selection markers, reporter genes, and two different promoters.
Table 1. Available Lenti-Labeler Constructs![](https://cdn11.bigcommerce.com/s-i3n9sxgjum/product_images/uploaded_images/moved-127f05b8b80a140ba292b70e22285810.png)
Supporting Data
SBI’s Lenti-Labelers get your cells labeled efficiently, reliably
Figure 1. SBI Lenti-Labeler constructs reliably and efficiently label cells. Comparison of the number of fluorescently-labeled cells to the total number of cells seen in the corresponding phase contrast images reveals the high labeling efficiency of SBI’s Lenti-Labeler constructs.
Figure 2. Dual reporter Lenti-Labeler vectors deliver robust fluorescence (A) and luminescence (B) signals. Data was obtained three days after infection of MDA-MB-231 cells with the dual reporter Lenti-Labeler Lentivirus CMV-rFLuc-T2A-GFP-mPGK-Puro (Cat #LL310VA-1). Infection efficiency was maximized by using SBI’s PEG-it Reagent (Cat.# LV810A-1) to concentrate the Lenti-Labeler Lentivirus prior to infection.