System Biosciences

pLL-EF1a-rFLuc-T2A-mRFP-mPGK-Puro [Lenti-Labeler™ virus]

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SKU:: LL420VA-1
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Description

Description

pLL-EF1a-rFLuc-T2A-mRFP-mPGK-Puro [Lenti-LabelerTM virus]. Cat# LL420VA. Supplier: SBI System Biosciences

Reliably co-label cells with rFLuc and mRFP driven by a moderate EF1α promoter (and with puro selection) - great for tracking cells, HTS assays, and more

Proven—leverages SBI’s third generation lentivector technology for optimal virus titers
Validated—sequence-verified from LTR to LTR and expression-verified in HT-1080 cells
Flexible—available in a range of fluorescent markers, selection markers, and promoters (Table 1)

Products

Overview

Reliable cell labeling, delivered SBI’s family of Lenti-Labeler™ constructs facilitate a wide range of studies—including cell tracking, high-throughput assays, and more—by enabling efficient and reliable labeling of your cells. The pLL-EF1a-rFLuc-T2A-mRFP-mPGK-Puro Lenti-Labeler construct co-expresses rFLuc and mRFP from the EF1α promoter, which delivers moderate expression in most cell types (including primary and stem cells), and co-expresses the puromycin resistance gene for selection in vitro prior to in vivo use. Available as either fully propagatable, sequence-verified plasmid DNA (Cat.# LL420PA-1) or ready-to-transduce pre-packaged lentivirus (Cat.# LL420VA-1), SBI’s pLL-EF1a-rFLuc-T2A-mRFP-mPGK-Puro Lenti-Labeler is designed for reliability, so you can get to valuable insights faster

Proven—leverages SBI’s third generation lentivector technology for optimal virus titers
Validated—sequence-verified from LTR to LTR and expression-verified in HT-1080 cells
Flexible—available in a range of fluorescent markers, selection markers, and promoters (Table 1)

While the pLL-EF1a-rFLuc-T2A-mRFP-mPGK-Puro Lenti-Labeler construct is compatible with most second and third generation packaging plasmid mixes, SBI recommends the use of pPACKH1 (Cat #LV500A-1) and TransDux MAX Transduction Reagent (Cat #LV860A-1) to achieve optimal virus titers and infection of target cells.Find the right Lenti-Labeler construct for your studies

SBI offers Lenti-Labeler constructs with a range of selection markers, reporter genes, and two different promoters.

Table 1. Available Lenti-Labeler Constructs

Supporting Data

SBI’s Lenti-Labelers get your cells labeled efficiently, reliably

Figure 1. SBI Lenti-Labeler constructs reliably and efficiently label cells. Comparison of the number of fluorescently-labeled cells to the total number of cells seen in the corresponding phase contrast images reveals the high labeling efficiency of SBI’s Lenti-Labeler constructs.

Figure 2. Dual reporter Lenti-Labeler vectors deliver robust fluorescence (A) and luminescence (B) signals. Data was obtained three days after infection of MDA-MB-231 cells with the dual reporter Lenti-Labeler Lentivirus CMV-rFLuc-T2A-GFP-mPGK-Puro (Cat #LL310VA-1). Infection efficiency was maximized by using SBI’s PEG-it Reagent (Cat.# LV810A-1) to concentrate the Lenti-Labeler Lentivirus prior to infection.