pSIH1-H1-Puro shRNA Cloning and Expression Vector

Using SBI’s shRNA lentivectors to produce siRNAs

To produce siRNAs for RNAi using the pSIF1-H1-copGFP Cloning and Expression Lentivector, first clone your shRNA template into the unique BamHI or EcoRI sites in the vector. After packaging and transduction, the vector will integrate into the genome and your shRNA will be transcribed from the H1 promoter using RNA polymerase III. The shRNA is transcribed as a single strand with a sense-loop-anti-sense structure that folds into a hairpin, and is then processed by DICER to produce an active siRNA molecule (Figure 1).

Figure 1. Generating siRNA from the pSIH1-H1-Puro Cloning and Expression Lentivector.