Regulatory RNA qPCR Profiler
- Usually Shipped in 5 Working Days
- 20 rxn
- Shipping Temperature:
- Blue Ice
Regulatory RNA qPCR Profiler. Cat# RA950A. Supplier: SBI System Biosciences
Understand the roles of regulatory RNAs with this kit that includes cDNA synthesis reagents and primer sets for qPCR analysis of 89 regulatory RNAs
Profile 89 regulatory RNAs with this kit that includes reagents for converting RNAs to cDNAs and a primer set for qPCR assays in a 96-well format. All primers have been validated against total RNA from several cell lines and tissues such as HEK293, HT1080, MCF7 (human breast cancer cell line), human fibroblast cells, human fetal brain, human adult brain, iPSC cells, and neural precursor cells (NPCs).
The kit includes four RNA controls for normalization—GAPDH, Actin, U6, and 18SrRNA—as well as assays for FOXO3 and BRD4 to detect eRNAs.
How It Works
Using the Regulatory RNA qPCR Profiler
First, get your regulatory RNA ready for qPCR by converting to cDNA
While some lncRNAs have endogenouse polyA tails, other lncRNAs do not. To enhance qPCR assay performance, the cDNA synthesis workflow includes steps and reagents to polyadenylate all lncRNAs before cDNA conversion using the tagged oligo dT adaptor and random primers. This combined RNA tailing and oligo dT plus random primers boosts cDNA yield significantly and enables strand-specific lncRNA qPCR profiling.
- Tag all lncRNAs with a poly-A tail
- Anneal an oligo-dT adaptor
- Reverse transcribe to create first-strand cDNA
The result is a cDNA pool of anchor-tailed lncRNAs that are ready for qPCR.
Then conduct qPCR assays
Get additional information about the array including RNA sequences, database links, and qPCR analysis templates in the Excel document,
Example Regulatory RNA qPCR Profiler Data