Reporters

Reporters

Reporters

pGreenFire 2.0 mCMV Cloning & Negative Control Lentivector & Virus


With the pGreenFire 2.0 mCMV vector you can add-in your own transcriptional response elements or use as-is as a negative control for any pGreenFire 2.0 project.

Make your own pGreenFire 2.0 reporter vector or use as a negative control

  1. With the pGreenFire 2.0 mCMV Cloning & Negative Control Lentivector and LentiVirus (pGF2-mCMV-rFluc-T2A-GFP-mPGK-Puro), you can take advantage of our robust pGreenFire 2.0 lentivector technology to create your own transcriptional response element (TRE) reporter or use pGreenFire 2.0 mCMV as-is as a negative control.
  2. The pGreenFire 2.0 mCMV Cloning & Negative Control Lentivector, XhoI and NheI sites are placed upstream of a minimal CMV promoter (mCMV) so you can clone in your own TREs. Upon activation, the TREs and mCMV promoter together drive co-expression of red firefly luciferase and GFP so you can quantitatively measure transcriptional activity using both fluorescence and luciferase activity.
  3. Alternatively, you can use the vector as-is as a negative control for any project using pGreenFire 2.0 lentivectors (see data below).
  4. What makes our next-gen pGreenFire 2.0 vectors even better than other TRE reporter vectors is the smart design, which adds in a constitutive selection cassette for stable cell line generation while minimizing interference with the upstream TRE. By using a weak/moderate mPGK promoter to drive the antibiotic selection marker (puromycin resistance) and carefully arranging the conditional reporter genes, the selection marker is reliably expressed without compromising conditional expression of rFLuc and GFP.
  5. As with all of our pGreenFire 2.0 lentivectors, the GreenFire cassette now consists of red firefly luciferase (rFLuc), a T2A co-expression element, and GFP. The switch to rFLuc opens up the possibility of performing a dual-spectral luciferase assay and also delivers greater sensitivity for in vivo applications than conventional luciferase.

Prodotti e Servizi dell'impresa ABM Inc.

 

  • Product
  • Qty in Cart
  • Quantity
  • Price
  • Subtotal
  • pGreenFire 2.0-mCMV negative control virus (pGF2-mCMV-rFluc-T2A-GFP-mPGK-Puro) pGreenFire 2.0-mCMV negative control virus (pGF2-mCMV-rFluc-T2A-GFP-mPGK-Puro)

    pGreenFire 2.0-mCMV negative control virus (pGF2-mCMV-rFluc-T2A-GFP-mPGK-Puro)

    System Biosciences

    zł3,412.10
    pGreenFire 2.0-mCMV negative control virus (pGF2-mCMV-rFluc-T2A-GFP-mPGK-Puro). Cat# TR411VA-P. Supplier: SBI System Biosciences With the pGreenFire 2.0 mCMV vector you can add-in your own transcriptional response elements or use as-is as a negative...
    zł3,412.10
    Qty in Cart: 0
    Quantity:
    Price:
    zł3,412.10
    Subtotal:
  • pGreenFire 2.0-mCMV negative control plasmid (pGF2-mCMV-rFluc-T2A-GFP-mPGK-Puro) pGreenFire 2.0-mCMV negative control plasmid (pGF2-mCMV-rFluc-T2A-GFP-mPGK-Puro)

    pGreenFire 2.0-mCMV negative control plasmid (pGF2-mCMV-rFluc-T2A-GFP-mPGK-Puro)

    System Biosciences

    zł3,412.10
    pGreenFire 2.0-mCMV negative control plasmid (pGF2-mCMV-rFluc-T2A-GFP-mPGK-Puro). Cat# TR411PA-P. Supplier: SBI System Biosciences With the pGreenFire 2.0 mCMV vector you can add-in your own transcriptional response elements or use as-is as a negative...
    zł3,412.10
    Qty in Cart: 0
    Quantity:
    Price:
    zł3,412.10
    Subtotal:
  • pGreenFire 2.0-CMV positive control virus (pGF2-CMV-rFluc-T2A-GFP-mPGK-Puro) pGreenFire 2.0-CMV positive control virus (pGF2-CMV-rFluc-T2A-GFP-mPGK-Puro)

    pGreenFire 2.0-CMV positive control virus (pGF2-CMV-rFluc-T2A-GFP-mPGK-Puro)

    System Biosciences

    zł3,412.10
    pGreenFire 2.0-CMV positive control virus (pGF2-CMV-rFluc-T2A-GFP-mPGK-Puro). Cat# TR410VA-P. Supplier: SBI System Biosciences Use the pGreenFire 2.0 CMV Positive Control Lentivector as a ready-to-go positive control for any pGreenFire 2.0 project...
    zł3,412.10
    Qty in Cart: 0
    Quantity:
    Price:
    zł3,412.10
    Subtotal:
  • pGreenFire 2.0-CMV positive control plasmid (pGF2-CMV-rFluc-T2A-GFP-mPGK-Puro) pGreenFire 2.0-CMV positive control plasmid (pGF2-CMV-rFluc-T2A-GFP-mPGK-Puro)

    pGreenFire 2.0-CMV positive control plasmid (pGF2-CMV-rFluc-T2A-GFP-mPGK-Puro)

    System Biosciences

    zł3,412.10
    pGreenFire 2.0-CMV positive control plasmid (pGF2-CMV-rFluc-T2A-GFP-mPGK-Puro). Cat# TR410PA-P. Supplier: SBI System Biosciences Use the pGreenFire 2.0 CMV Positive Control Lentivector as a ready-to-go positive control for any pGreenFire 2.0 project...
    zł3,412.10
    Qty in Cart: 0
    Quantity:
    Price:
    zł3,412.10
    Subtotal:
  • pGreenFire 2.0 TCF/LEF reporter virus (pGF2-TCF/LEF-rFluc-T2A-GFP-mPGK-Puro) pGreenFire 2.0 TCF/LEF reporter virus (pGF2-TCF/LEF-rFluc-T2A-GFP-mPGK-Puro)

    pGreenFire 2.0 TCF/LEF reporter virus (pGF2-TCF/LEF-rFluc-T2A-GFP-mPGK-Puro)

    System Biosciences

    zł3,787.66
    pGreenFire 2.0 TCF/LEF reporter virus (pGF2-TCF/LEF-rFluc-T2A-GFP-mPGK-Puro). Cat# TR413VA-P. Supplier: SBI System Biosciences Study TCF/LEF signaling with pGreenFire 2.0 TCF/LEF Reporter (red firefly luciferase & GFP) – specially engineered...
    zł3,787.66
    Qty in Cart: 0
    Quantity:
    Price:
    zł3,787.66
    Subtotal:
  • pGreenFire 2.0 TCF/LEF reporter plasmid (pGF2-TCF/LEF-rFluc-T2A-GFP-mPGK-Puro) pGreenFire 2.0 TCF/LEF reporter plasmid (pGF2-TCF/LEF-rFluc-T2A-GFP-mPGK-Puro)

    pGreenFire 2.0 TCF/LEF reporter plasmid (pGF2-TCF/LEF-rFluc-T2A-GFP-mPGK-Puro)

    System Biosciences

    zł3,787.66
    pGreenFire 2.0 TCF/LEF reporter plasmid (pGF2-TCF/LEF-rFluc-T2A-GFP-mPGK-Puro). Cat# TR413PA-P. Supplier: SBI System Biosciences Study TCF/LEF signaling with pGreenFire 2.0 TCF/LEF Reporter (red firefly luciferase & GFP) – specially engineered...
    zł3,787.66
    Qty in Cart: 0
    Quantity:
    Price:
    zł3,787.66
    Subtotal:
  • pGreenFire 2.0 TCF/LEF clonal 293T reporter cell line (pGF2-TCF/LEF-rFluc-T2A-GFP-mPGK-Puro) pGreenFire 2.0 TCF/LEF clonal 293T reporter cell line (pGF2-TCF/LEF-rFluc-T2A-GFP-mPGK-Puro)

    pGreenFire 2.0 TCF/LEF clonal 293T reporter cell line (pGF2-TCF/LEF-rFluc-T2A-GFP-mPGK-Puro)

    System Biosciences

    zł16,652.88
    pGreenFire 2.0 TCF/LEF clonal 293T reporter cell line (pGF2-TCF/LEF-rFluc-T2A-GFP-mPGK-Puro). Cat# TR413C-P. Supplier: SBI System Biosciences Study TCF/LEF signaling with pGreenFire 2.0 TCF/LEF Reporter (red firefly luciferase & GFP) –...
    zł16,652.88
    Qty in Cart: 0
    Quantity:
    Price:
    zł16,652.88
    Subtotal:
  • pGreenFire 2.0 NFkB reporter virus (pGF2-NFκB-rFluc-T2A-GFP-mPGK-Puro pGreenFire 2.0 NFkB reporter virus (pGF2-NFκB-rFluc-T2A-GFP-mPGK-Puro

    pGreenFire 2.0 NFkB reporter virus (pGF2-NFκB-rFluc-T2A-GFP-mPGK-Puro)

    System Biosciences

    zł3,787.66
    pGreenFire 2.0 NFkB reporter virus (pGF2-NFκB-rFluc-T2A-GFP-mPGK-Puro). Cat# TR412VA-P. Supplier: SBI System Biosciences Study NF-κB signaling with the pGreenFire 2.0 NFκB Reporter (red firefly luciferase & GFP), which is engineered...
    zł3,787.66
    Qty in Cart: 0
    Quantity:
    Price:
    zł3,787.66
    Subtotal:
  • pGreenFire 2.0 NFkB reporter plasmid (pGF2-NFκB-rFluc-T2A-GFP-mPGK-Puro pGreenFire 2.0 NFkB reporter plasmid (pGF2-NFκB-rFluc-T2A-GFP-mPGK-Puro

    pGreenFire 2.0 NFkB reporter plasmid (pGF2-NFκB-rFluc-T2A-GFP-mPGK-Puro)

    System Biosciences

    zł3,787.66
    pGreenFire 2.0 NFkB reporter plasmid (pGF2-NFκB-rFluc-T2A-GFP-mPGK-Puro). Cat# TR412PA-P. Supplier: SBI System Biosciences Study NF-κB signaling with the pGreenFire 2.0 NFκB Reporter (red firefly luciferase & GFP), which is...
    zł3,787.66
    Qty in Cart: 0
    Quantity:
    Price:
    zł3,787.66
    Subtotal:
  • pGreenFire 2.0 NFAT reporter virus (pGF2-NFAT-rFluc-T2A-GFP-mPGK-Puro) pGreenFire 2.0 NFAT reporter virus (pGF2-NFAT-rFluc-T2A-GFP-mPGK-Puro)

    pGreenFire 2.0 NFAT reporter virus (pGF2-NFAT-rFluc-T2A-GFP-mPGK-Puro)

    System Biosciences

    zł3,787.66
    pGreenFire 2.0 NFAT reporter virus (pGF2-NFAT-rFluc-T2A-GFP-mPGK-Puro). Cat# TR451VA-P. Supplier: SBI System Biosciences Study NFAT signaling with this next-gen pGreenFire 2.0 NFAT Reporter (red firefly luciferase & GFP) that is engineered for...
    zł3,787.66
    Qty in Cart: 0
    Quantity:
    Price:
    zł3,787.66
    Subtotal:
  • pGreenFire 2.0 NFAT reporter plasmid (pGF2-NFAT-rFluc-T2A-GFP-mPGK-Puro) pGreenFire 2.0 NFAT reporter plasmid (pGF2-NFAT-rFluc-T2A-GFP-mPGK-Puro)

    pGreenFire 2.0 NFAT reporter plasmid (pGF2-NFAT-rFluc-T2A-GFP-mPGK-Puro)

    System Biosciences

    zł3,787.66
    pGreenFire 2.0 NFAT reporter plasmid (pGF2-NFAT-rFluc-T2A-GFP-mPGK-Puro). Cat# TR451PA-P. Supplier: SBI System Biosciences Study NFAT signaling with this next-gen pGreenFire 2.0 NFAT Reporter (red firefly luciferase & GFP) that is engineered for...
    zł3,787.66
    Qty in Cart: 0
    Quantity:
    Price:
    zł3,787.66
    Subtotal:
  • pGreenFire 2.0 HIF-1 reporter virus (pGF2-HIF1-rFluc-T2A-GFP-mPGK-Puro) pGreenFire 2.0 HIF-1 reporter virus (pGF2-HIF1-rFluc-T2A-GFP-mPGK-Puro)

    pGreenFire 2.0 HIF-1 reporter virus (pGF2-HIF1-rFluc-T2A-GFP-mPGK-Puro)

    System Biosciences

    zł3,787.66
    pGreenFire 2.0 HIF-1 reporter virus (pGF2-HIF1-rFluc-T2A-GFP-mPGK-Puro). Cat# TR426VA-P. Supplier: SBI System Biosciences Study HIF-1 signaling with the pGreenFire 2.0 HIF-1 Reporter (red firefly luciferase & GFP) which is engineered for reliable...
    zł3,787.66
    Qty in Cart: 0
    Quantity:
    Price:
    zł3,787.66
    Subtotal:
  • pGreenFire 2.0 HIF-1 reporter plasmid (pGF2-HIF1-rFluc-T2A-GFP-mPGK-Puro) pGreenFire 2.0 HIF-1 reporter plasmid (pGF2-HIF1-rFluc-T2A-GFP-mPGK-Puro)

    pGreenFire 2.0 HIF-1 reporter plasmid (pGF2-HIF1-rFluc-T2A-GFP-mPGK-Puro)

    System Biosciences

    zł3,787.66
    pGreenFire 2.0 HIF-1 reporter plasmid (pGF2-HIF1-rFluc-T2A-GFP-mPGK-Puro). Cat# TR426PA-P. Supplier: SBI System Biosciences Study HIF-1 signaling with the pGreenFire 2.0 HIF-1 Reporter (red firefly luciferase & GFP) which is engineered for reliable...
    zł3,787.66
    Qty in Cart: 0
    Quantity:
    Price:
    zł3,787.66
    Subtotal:
  • pGreenFire 2.0 Estrogen response element reporter virus (pGF2-ERE-rFluc-T2A-GFP-mPGK-Puro) pGreenFire 2.0 Estrogen response element reporter virus (pGF2-ERE-rFluc-T2A-GFP-mPGK-Puro)

    pGreenFire 2.0 Estrogen response element reporter virus (pGF2-ERE-rFluc-T2A-GFP-mPGK-Puro)

    System Biosciences

    zł3,787.66
    pGreenFire 2.0 Estrogen response element reporter virus (pGF2-ERE-rFluc-T2A-GFP-mPGK-Puro). Cat# TR455VA-P. Supplier: SBI System Biosciences Study estrogen signaling with this next-gen pGreenFire 2.0 lentivector (red firefly luciferase & GFP), which...
    zł3,787.66
    Qty in Cart: 0
    Quantity:
    Price:
    zł3,787.66
    Subtotal:
  • pGreenFire 2.0 Estrogen response element reporter plasmid (pGF2-ERE-rFluc-T2A-GFP-mPGK-Puro) pGreenFire 2.0 Estrogen response element reporter plasmid (pGF2-ERE-rFluc-T2A-GFP-mPGK-Puro)

    pGreenFire 2.0 Estrogen response element reporter plasmid (pGF2-ERE-rFluc-T2A-GFP-mPGK-Puro)

    System Biosciences

    zł3,787.66
    pGreenFire 2.0 Estrogen response element reporter plasmid (pGF2-ERE-rFluc-T2A-GFP-mPGK-Puro). Cat# TR455PA-P. Supplier: SBI System Biosciences Study estrogen signaling with this next-gen pGreenFire 2.0 lentivector (red firefly luciferase & GFP),...
    zł3,787.66
    Qty in Cart: 0
    Quantity:
    Price:
    zł3,787.66
    Subtotal:
  • pGreenFire 2.0 AP-1 reporter virus (pGF2-AP1-rFluc-T2A-GFP-mPGK-Puro) pGreenFire 2.0 AP-1 reporter virus (pGF2-AP1-rFluc-T2A-GFP-mPGK-Puro)

    pGreenFire 2.0 AP-1 reporter virus (pGF2-AP1-rFluc-T2A-GFP-mPGK-Puro)

    System Biosciences

    zł3,787.66
    pGreenFire 2.0 AP-1 reporter virus (pGF2-AP1-rFluc-T2A-GFP-mPGK-Puro). Cat# TR452VA-P. Supplier: SBI System Biosciences Study oncogenic signaling with the next-gen pGreenFire 2.0 AP-1 Reporter (red firefly luciferase & GFP), engineered for reliable...
    zł3,787.66
    Qty in Cart: 0
    Quantity:
    Price:
    zł3,787.66
    Subtotal:
  • pGreenFire 2.0 AP-1 reporter plasmid (pGF2-AP1-rFluc-T2A-GFP-mPGK-Puro) pGreenFire 2.0 AP-1 reporter plasmid (pGF2-AP1-rFluc-T2A-GFP-mPGK-Puro)

    pGreenFire 2.0 AP-1 reporter plasmid (pGF2-AP1-rFluc-T2A-GFP-mPGK-Puro)

    System Biosciences

    zł3,787.66
    pGreenFire 2.0 AP-1 reporter plasmid (pGF2-AP1-rFluc-T2A-GFP-mPGK-Puro). Cat# TR452PA-P. Supplier: SBI System Biosciences Study oncogenic signaling with the next-gen pGreenFire 2.0 AP-1 Reporter (red firefly luciferase & GFP), engineered for reliable...
    zł3,787.66
    Qty in Cart: 0
    Quantity:
    Price:
    zł3,787.66
    Subtotal:
  • pGreenFire 2.0 AP-1 clonal 293T reporter cell line (pGF2-AP1-rFluc-T2A-GFP-mPGK-Puro) pGreenFire 2.0 AP-1 clonal 293T reporter cell line (pGF2-AP1-rFluc-T2A-GFP-mPGK-Puro)

    pGreenFire 2.0 AP-1 clonal 293T reporter cell line (pGF2-AP1-rFluc-T2A-GFP-mPGK-Puro)

    System Biosciences

    zł16,652.88
    pGreenFire 2.0 AP-1 clonal 293T reporter cell line (pGF2-AP1-rFluc-T2A-GFP-mPGK-Puro). Cat# TR452C-P. Supplier: SBI System Biosciences Study oncogenic signaling with the next-gen pGreenFire 2.0 AP-1 Reporter (red firefly luciferase & GFP), engineered...
    zł16,652.88
    Qty in Cart: 0
    Quantity:
    Price:
    zł16,652.88
    Subtotal:
  • Total: items /

Adding your products to cart