System Biosciences

XPack CMV-XP-MCS-EF1-Puro Cloning Lentivector

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SKU:
XPAK510PA-1
Availability:
Usually Shipped in 5 Working Days
Size:
10 ug
Shipping Temperature:
Blue Ice/ Dry Ice
  • XPack CMV-XP-MCS-EF1-Puro Cloning Lentivector
  • XPack CMV-XP-MCS-EF1-Puro Cloning Lentivector
€861.00

Description

XPack CMV-XP-MCS-EF1-Puro Cloning Lentivector. Cat# XPAK510PA. Supplier: SBI System Biosciences

Package your protein-of-interest into exosomes with the XPack CMV-XP-MCS-EF1α-Puro Cloning Lentivector—great for delivering protein to target cells and more.
  • Powerful—enables a wealth of applications, including Exosome labeling
    Exosome cargo tracking
    Exosomes as cargo delivery vehicles
    Exosomes as biotherapeutics
    Exosome-generating stable cell lines with protein cargo-of-interest
  • Exosome labeling
  • Exosome cargo tracking
  • Exosomes as cargo delivery vehicles
  • Exosomes as biotherapeutics

Products

Overview

Turn your protein into exosome cargo with XPack™

Whether you’re engineering exosomes as protein delivery vehicles, labeling exosomes with a custom reporter protein, or performing functional studies, XPack gives you the power to package your protein-of-interest into exosomes.

XPack CMV-XP-MCS-EF1α-Puro Cloning Lentivector

With the XPack CMV-XP-MCS-EF1α-Puro Cloning Lentivector, you can use SBI’s well-proven lentivector technology to express your protein-of-interest and package it into exosomes. The strong CMV promoter delivers high expression levels in most cell lines and the multiple cloning site downstream of the XPack tag enables fusion of the exosome-targeting peptide to your protein-of-interest (also available with an MSCV promoter for expression in hematopoietic and stem cells).

The XPack System at a glance:

  • Powerful—enables a wealth of applications, including
    • Exosome labeling
    • Exosome cargo tracking
    • Exosomes as cargo delivery vehicles
    • Exosomes as biotherapeutics
    • Exosome-generating stable cell lines with protein cargo-of-interest
  • Optimized—SBI developed the XPack peptide tag for robust exosome targeting
  • Reliable—based on SBI’s highly-cited, well-proven lentivector technology
  • Versatile
    • Two promoter options for expression in a wide range of cells
    • Comes as pre-built reporters (GFP, RFP, luciferase) or ready-to-customize cloning lentivectors

Choose the XPack Lentivector that’s right for you

How It Works

Optimized for success

With XPack, SBI has developed an optimized N-terminal peptide sequence that targets the peptide—and any protein fused to it—to the inner surface of the exosome membrane. Clone your protein of interest into one of the XPack Cloning Vectors and, when expressed, your protein will be packaged into exosomes.

Supporting Data

Engineer exosomes with XPack and deliver functional protein to cells, changing cellular physiology

Cyclin-dependent kinase inhibitor 1B (CDKN1B) is a 22 KDa protein which is a cell cycle inhibitor and tumor suppressor. To demonstrate how exosomes can be used to deliver functional protein to cells, we generated exosomes containing CDKN1B and show that addition of these exosomes HEK-293 cells can reduce cellular proliferation (Figure 1).

 

Figure 1. XPack-CDKN1B exosomes reduce cellular proliferation. METHODS:

We cloned the CDKN1B gene into CMV-XP-MCS-EF1α-Puro Cloning Lentivector (Cat.# XPAK510PA-1), transfected this construct into HEK293 cells, and isolated exosomes after 48 hours using ExoQuick-TC®. We then added 5, 50, or 100 µg of XPack-CDKN1B exosomes to approximately 5000 HEK-293 target cells in 96-well tissue culture plates at 24-hour intervals over the course of 72 hours. Exosomes isolated from non-transfected cells were used for controls. Cellular proliferation was quantified using an MTT Assay (Millipore catalog # CT02) and normalilzed to HEK-293 cells treated with control exosomes. XPack-CDKN1B exosomes consistently reduce the rate of cellular proliferation.

 

 

 
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