System Biosciences
XPack CMV-XP-MCS-EF1-Puro Cloning Lentivector
- SKU:
- XPAK510PA-1
- UPC:
- MPN:
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 10 ug
- Shipping Temperature:
- Blue Ice/ Dry Ice
Description
XPack CMV-XP-MCS-EF1-Puro Cloning Lentivector. Cat# XPAK510PA. Supplier: SBI System Biosciences
- Powerful—enables a wealth of applications, including Exosome labeling
Exosome cargo tracking
Exosomes as cargo delivery vehicles
Exosomes as biotherapeutics
Exosome-generating stable cell lines with protein cargo-of-interest - Exosome labeling
- Exosome cargo tracking
- Exosomes as cargo delivery vehicles
- Exosomes as biotherapeutics
Products

Overview
Whether you’re engineering exosomes as protein delivery vehicles, labeling exosomes with a custom reporter protein, or performing functional studies, XPack gives you the power to package your protein-of-interest into exosomes.
XPack CMV-XP-MCS-EF1α-Puro Cloning Lentivector
With the XPack CMV-XP-MCS-EF1α-Puro Cloning Lentivector, you can use SBI’s well-proven lentivector technology to express your protein-of-interest and package it into exosomes. The strong CMV promoter delivers high expression levels in most cell lines and the multiple cloning site downstream of the XPack tag enables fusion of the exosome-targeting peptide to your protein-of-interest (also available with an MSCV promoter for expression in hematopoietic and stem cells).
The XPack System at a glance:
- Powerful—enables a wealth of applications, including
- Exosome labeling
- Exosome cargo tracking
- Exosomes as cargo delivery vehicles
- Exosomes as biotherapeutics
- Exosome-generating stable cell lines with protein cargo-of-interest
- Optimized—SBI developed the XPack peptide tag for robust exosome targeting
- Reliable—based on SBI’s highly-cited, well-proven lentivector technology
- Versatile
- Two promoter options for expression in a wide range of cells
- Comes as pre-built reporters (GFP, RFP, luciferase) or ready-to-customize cloning lentivectors
Choose the XPack Lentivector that’s right for you
How It Works
Optimized for success
With XPack, SBI has developed an optimized N-terminal peptide sequence that targets the peptide—and any protein fused to it—to the inner surface of the exosome membrane. Clone your protein of interest into one of the XPack Cloning Vectors and, when expressed, your protein will be packaged into exosomes.
Supporting Data
Engineer exosomes with XPack and deliver functional protein to cells, changing cellular physiology
Cyclin-dependent kinase inhibitor 1B (CDKN1B) is a 22 KDa protein which is a cell cycle inhibitor and tumor suppressor. To demonstrate how exosomes can be used to deliver functional protein to cells, we generated exosomes containing CDKN1B and show that addition of these exosomes HEK-293 cells can reduce cellular proliferation (Figure 1).
Figure 1. XPack-CDKN1B exosomes reduce cellular proliferation. METHODS:
We cloned the CDKN1B gene into CMV-XP-MCS-EF1α-Puro Cloning Lentivector (Cat.# XPAK510PA-1), transfected this construct into HEK293 cells, and isolated exosomes after 48 hours using ExoQuick-TC®. We then added 5, 50, or 100 µg of XPack-CDKN1B exosomes to approximately 5000 HEK-293 target cells in 96-well tissue culture plates at 24-hour intervals over the course of 72 hours. Exosomes isolated from non-transfected cells were used for controls. Cellular proliferation was quantified using an MTT Assay (Millipore catalog # CT02) and normalilzed to HEK-293 cells treated with control exosomes. XPack-CDKN1B exosomes consistently reduce the rate of cellular proliferation.
Related Products


XPack CMV-XP-GFP-EF1-Puro Expression Lentivector
System Biosciences


XPack CMV-XP-Luciferase-EF1-Puro Expression Lentivector
System Biosciences


XPack CMV-XP-RFP-EF1-Puro Expression Lentivector
System Biosciences


XPack MSCV-XP-Luciferase-EF1-Puro Expression Lentivector
System Biosciences


XPack MSCV-XP-MCS-EF1-Puro Cloning Lentivector
System Biosciences
Customers Also Viewed


XStamp cloning and expression lentivector MSCV-Leader-MCS-C1C2-EF1-Puro
System Biosciences


XMIRXpress cloning lentivector with Xmotif
System Biosciences


pSIH1-H1-Puro shRNA Cloning and Expression Vector
System Biosciences


XPack MSCV-XP-MCS-EF1-Puro Cloning Lentivector
System Biosciences


pCDH-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector
System Biosciences


pCDF1-MCS2-EF1-Puro cDNA Cloning and Expression Lentivector
System Biosciences


PB-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector
System Biosciences


PB-Cuo-MCS-IRES-GFP-EF1-CymR-Puro Inducible cDNA Cloning and Expression Vector
System Biosciences


Exo-FITC Exosome FACS stain
System Biosciences

h NFkB1 inducible lentiviral particles | LVP210
GenTarget


XStamp MLN exosome Motilin receptor GI tract targeting lentivector
System Biosciences


XPack CMV-XP-Luciferase-EF1-Puro Expression Lentivector
System Biosciences


XPack CMV-XP-GFP-EF1-Puro Expression Lentivirus
System Biosciences


XCF COMPLETE Exosome and cfDNA Isolation Kit (for Serum and Plasma)
System Biosciences


Water-soluble Cumate solution (300mg/ml, 10,000x), 500ul
System Biosciences


Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL)
System Biosciences


Super piggyBac Transposase expression vector (replaces PB200A-1), 50 rxns
System Biosciences


SmartSEC Single EV Isolation System
System Biosciences


SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns)
System Biosciences


RAWS 264.7 Mouse macrophage cell line: >1x10^6 frozen exosomes
System Biosciences


pMSCV-Mouse Foxp3-EF1-GFP-T2A-Puro piggyBac transposon plasmid
System Biosciences


pMSCV-Human RORgt-EF1-GFP-T2A-Puro piggyBac transposon plasmid
System Biosciences


pMSCV-Human Foxp3-EF1-GFP-T2A-Puro piggyBac transposon plasmid
System Biosciences
![piggyBac-HR with GFP+Puro markers and TK selection [MCS1-5'PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3' PB TR-MCS2] for Gene Correctio piggyBac-HR with GFP+Puro markers and TK selection [MCS1-5'PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3' PB TR-MCS2] for Gene Correctio](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/14421/14689/sbi%20system%20biosciences_1633075021__00205.original__16600.1633076840.jpg?c=1)
![piggyBac-HR with GFP+Puro markers and TK selection [MCS1-5'PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3' PB TR-MCS2] for Gene Correctio piggyBac-HR with GFP+Puro markers and TK selection [MCS1-5'PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3' PB TR-MCS2] for Gene Correctio](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/14421/29947/PBHR100A-1__86845.1640081714.png?c=1)
piggyBac-HR with GFP+Puro markers and TK selection [MCS1-5'PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3' PB TR-MCS2] for Gene Correction
System Biosciences


pCDH-UbC-MCS-IRES-GFP cDNA Cloning and Expression Vector
System Biosciences


pCDH-EF1-MCS-IRES-Neo cDNA Cloning and Expression Vector
System Biosciences


pCDH-EF1-MCS-(PGK-GFP-T2A-Puro) Cloning and Expression Vector
System Biosciences


pCDH-CMV-MCS-EF1-RFP cDNA Cloning and Expression Vector
System Biosciences


pCDH-CMV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector
System Biosciences


PC-3 Human prostate cancer cells derived from metastatic cancer cell line: >1x10^10 frozen exosomes
System Biosciences


PB-EF1-MCS-IRES-Neo cDNA cloning and expression vector
System Biosciences


PB-EF1-GreenPuro-H1-MCS shRNA cloning and expression vector
System Biosciences


PB-CMV-MCS-EF1-RedPuro cDNA Cloning and Expression Vector
System Biosciences


PB-CMV-MCS-EF1-Puro cDNA cloning and expression vector
System Biosciences


PB-CMV-GreenPuro Scramble Hairpin Control Vector
System Biosciences


pAAVK-EF1α-MCS1-CMV-mRFP AAVanced™ Cloning and Expression Vector
System Biosciences

miRZip pooled anti-miR virus library (Pre-packaged) >10^7 IFUs/aliquot
System Biosciences


HEK293 Human embryonic kidney cell line: >1x10^10 frozen exosomes
System Biosciences


H841 Human small cell lung cancer cell line: >1x10^10 frozen exosomes
System Biosciences


H196 Human small cell lung cancer cell line: >1x10^10 frozen exosomes
System Biosciences


Exosome-Depleted FBS Media Supplement
System Biosciences


ExoQuick-TC PLUS Exosome Purification Kit - for tissue culture media
System Biosciences


ExoQuick ULTRA EV Isolation Kit for Serum and Plasma
System Biosciences


ExoQuick PLUS Exosome Purification Kit - for serum and plasma
System Biosciences


ExoQuick Exosome RNA Column Purification kit
System Biosciences


ExoQuick Exosome Isolation and RNA Purification kit (for Tissue Culture Media)
System Biosciences


ExoMS Surface Protein Capture Kit (Tissue Culture EVs)
System Biosciences


Excision only piggyBac Transposase expression vector, 50 rxns
System Biosciences


EVeryRNA™ EV RNA Purification System with SmartSEC Single (10 reactions)
System Biosciences


EV Shuttle Kit Mouse JAWS II Mouse bone marrow dendritic cell exosomes (10 rxn)
System Biosciences