EV Shuttle Kit Human HEK293 cell exosomes (10 rxn)

To demonstrate the excellent RNA yields and robust cDNA synthesis obtained with EVeryRNA, we isolated EVs from 250 µL of serum using SmartSEC Single, spiked in 0.1 pmol of Cel-miR-39, and used both the EVeryRNA EV Purification System and a phenol-based kit to isolate RNA. The isolated RNA was reverse transcribed using the EVeryRNA cDNA Synthesis & Pre-amplification Kit and the copy number of Cel-miR-39 measured (Figure 1). The EVeryRNA EV Purification System delivered similar levels of Cel-miR-39 as the phenol-based method.

EVeryRNA efficiently isolates mRNA

We used the EVeryRNA EV RNA Purification System and EVeryRNA cDNA Synthesis & Pre-amplification Kit to isolate mRNA and synthesize cDNA from cells overexpressing eGFP (Figure 2). Robust levels of eGFP mRNA are recovered and converted to cDNA when cells are overexpressing eGFP.

miRNA isolated and converted to cDNA using EVeryRNA can be used for miRNA profiling

We isolated EVs from 250 µL of serum using SmartSEC Single and used the EVeryRNA EV RNA Purification System and EVeryRNA cDNA Synthesis & Pre-amplification System to isolate and reverse transcribe EV RNAs for miRNA profiling using the SeraMir Human Exosome RNA Profiling Plate. We were able to detect a number of miRNAs both with amplification (96 miRNAs) and without amplification (16 miRNAs, with 14 overlapping with the miRNAs detected with amplification, Figure 3).