EV Shuttle Kit Mouse JAWS II Mouse bone marrow dendritic cell exosomes (5 rxn)
- Usually Shipped in 5 Working Days
- 5 reactions
- Shipping Temperature:
- Dry Ice
EV Shuttle Kit Mouse JAWS II Mouse bone marrow dendritic cell exosomes (5 rxn). Cat# EVS205A. Supplier: SBI System Biosciences
- Easy-to-use with a fast and straightforward loading protocol
- Introduces a wide range of biomolecules directly into isolated exosomes:
- RNAs, including siRNAs, miRNAs, and mRNAs
- DNAs, including plasmids
- Metabolites and other small molecules
- Enables non-viral transduction and stable cell line creation
- Provides an alternative gene delivery method for hard-to-transfect cells
How It Works
Quickly and easily load cargo into isolated exosomes
Simply combine the isolated exosomes provided in the EV Shuttle Kit with Exo-Fect Reagent (also included in the Kit) and the nucleic acid or small molecule of your choice to generate exosome delivery vehicles. The Kit comes with all of the reagents you need to load cargo into exosomes and concentrate them for delivery to target cells. The protocol takes less than an hour and is highly efficient at loading cargo into exosomes for transport and delivery.
The EV Shuttle Kit is also compatible with the EV-Entry Reagent, which maximizes cargo uptake by recipient cells.
See the EV Shuttle System deliver cargo and alter cellular physiology even in hard-to-transfect cells
The EV Shuttle System delivers functional siRNA
Figure 1. Mouse and Human EV Shuttles efficiently deliver siRNA to hard-to-transfect cells. (Left panel set) JAWS II mouse dendritic cell exosomes were incubated with Exo-Fect and 100 pmol Texas Red-conjugated siRNA (non-targeting control included in the EV Shuttle Kits). The siRNA-loaded EV Shuttles were then added to naive mouse monocyte macrophage cells (RAWS 264.7) in culture. The cells were imaged after 18 hours and delivery was observed as soon as 4 hours after adding the EV Shuttles. More than 80% of the recipient RAWS 264.7 cells internalized the EV Shuttle siRNA cargo.
(Right panel set) Human HEK 293 EV Shuttles were treated with SBI’s Exo-Green Kit (Cat.# EXOG200A-1) which fluorescently labels internal exosome proteins. The labeled Human EV Shuttles were then added to mouse embryonic stem cells and imaged for cargo delivery after 18 hours. The Human EV Shuttles were well-tolerated and the EVs were taken up efficiently by the mouse embryonic stem cells.
EV Shuttles can be used as a non-viral method for generating stable cell lines
Because plasmids can be transfected into exosomes with the EV Shuttle System, EV Shuttles can be used to generate stable cell lines without the use of viruses. Here we show an example using our non-viral PiggyBac Transposon System (Figure 4). Delivery of PiggyBac vectors by EV Shuttles was less toxic than lipofectamine-mediated transfection, resulted in highly efficient integration of the PB transposon vector into the host chromosome, and generated approximately 5-times more colonies than lipofectamine treatment (Figure 5).