SmartSEC™ Mini EV Isolation System
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SmartSEC™ Mini EV Isolation System. Cat# SSEC100A. Supplier: SBI System Biosciences</
Explore new frontiers in EV biology
Whether it’s precious clinical samples or hard-to-collect biofluids from developmental biology model organisms, researchers are increasingly challenged by the need to isolate EVs from quite limited sample volumes. To support these researchers, SBI has configured our powerful SmartSEC™ technology into the first commercially available kit optimized for isolating EVs from 10 – 100 µL of biofluid (Table 1). Validated for samples from Drosophila, Planaria, and Arabidopsis, as well as human, mouse, and rat, the SmartSEC™ Mini EV Isolation System is ready to help you push the boundaries of EV biology.
Table 1. SmartSEC Mini recommended input volumes
SmartSEC Mini is a proprietary chromatography-based EV isolation technology that combines all the benefits of size exclusion chromatography (SEC)—purity, yield, reproducibility, and preservation of EV integrity—with a contaminant trapping feature that overcomes the limitations of conventional SEC for fast, easy, and low sample-volume EV isolation.
- The only commercially available EV isolation kit for sample volumes as low as 10 µL
- Great for isolating EVs from a range of biofluids
- Validated in samples from human as well as model developmental biology organisms such as:
- Drosophila melanogaster
- Arabidopsis thaliana
- Delivers better purity and yield than ultracentrifugation
- Compatible with most downstream applications such as mass spectrometry, western blotting, nanoparticle tracking analysis (NTA), and transmission electron microscopy (TEM)
How It Works
See how well SmartSEC Mini isolates EVs from low volumes in a range of species, biofluidsSmartSEC Mini provides excellent purification of EVs from as little as 10 µL of hemolymph from Drosophila melanogaster, as demonstrated by western blot analysis of 1 µg of equivalent protein (Figure 2). Common Drosophila exosome markers are shown next to molecular weight markers.
Table 2. SmartSEC Mini delivers high yields of EVs
Table 3. SmartSEC Mini delivers more EVs than a competitor’s product
SmartSEC Mini specifically enriches for EVs versus lipoprotein particles and blood microparticles as demonstrated via mass spectrometry (Figure 6 and Table 4). We used the quick and easy SmartSEC Mini workflow to isolate EVs from 20 µL of human serum and analyzed the EV-associated proteins via mass spec. Of the ~600 proteins we identified (see Table 4 for a representative list), a functional enrichment analysis conducted using the FunRich tool shows that over 50% of the proteins are known to be associated with exosomes whereas <30% are associated with blood microparticles and <10% are associated with lipoprotein particles (Figure 6).
Table 4. Common EV-associated proteins identified from mass spec analysis of EVs isolated with SmartSEC Mini