- Home
- PiggyBac
- piggyBac-HR with GFP+Puro markers and TK selection [MCS1-5'PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3' PB TR-MCS2] for Gene Correction
System Biosciences
piggyBac-HR with GFP+Puro markers and TK selection [MCS1-5'PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3' PB TR-MCS2] for Gene Correction
- SKU:
- PBHR100A-1
- UPC:
- MPN:
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 10 ug
- Shipping Temperature:
- Blue Ice/ Dry Ice
- Qualifications:
- Requires signed license from commercial customers
Description
piggyBac-HR with GFP+Puro markers and TK selection [MCS1-5'PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3' PB TR-MCS2] for Gene Correction. Cat# PBHR100A. Supplier: SBI System Biosciences
Products

Overview
Use the PiggyBac Gene Editing HR Targeting Vector (MCS1-5'PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3' PB TR-MCS2) to get seamless gene editing—leave no trace of vector sequences behind.
This special HR Donor works with the Excision-only PiggyBac Transposase instead of the Cre-LoxP system. Simply proceed with your CRISPR/Cas9 gene editing as usual—clone your homology arms into MCS1 and MCS2, use dual GFP and puromycin selection to find integrants, and enrich for on-target events using negative thymidine kinase (TK) selection (Figure 1).
Why use an HR targeting vector?
Even though gene knock-outs can result from DSBs caused by Cas9 alone, SBI recommends the use of HR targeting vectors (also called HR donor vectors) for more efficient and precise mutation. HR donors can supply elements for positive or negative selection ensuring easier identification of successful mutation events. In addition, HR donors can include up to 6-8 kb of open reading frame for gene knock-ins or tagging, and, when small mutations are included in either 5’ or 3’ homology arms, can make specific, targeted gene edits.
Choose the right HR Targeting Vector for your project
How It Works

1 Review
-
5 superb
Posted by jimmy on 10th Jan 2023
PIGGYBAC-HR WITH GFP+PURO MARKERS AND TK SELECTION was a superb product and is recommended.
Related Products
![Gene Knock-Out HR Targeting Vector [MCS1-EF1a-GFP-T2A-Puro-pA-MCS2] Gene Knock-Out HR Targeting Vector [MCS1-EF1a-GFP-T2A-Puro-pA-MCS2]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/12671/12936/sbi%2520system%2520biosciences_1633075021__00205.original__02911.1633076564.jpg?c=1)
![Gene Knock-Out HR Targeting Vector [MCS1-EF1a-GFP-T2A-Puro-pA-MCS2] Gene Knock-Out HR Targeting Vector [MCS1-EF1a-GFP-T2A-Puro-pA-MCS2]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/12671/15641/Screenshot_2021-12-09_212928__74392.1639067413.png?c=1)
Gene Knock-Out HR Targeting Vector [MCS1-EF1a-GFP-T2A-Puro-pA-MCS2]
System Biosciences
![Gene Knock-Out HR Targeting Vector [MCS1-EF1α-RFP-T2A-Puro-pA-MCS2] Gene Knock-Out HR Targeting Vector [MCS1-EF1α-RFP-T2A-Puro-pA-MCS2]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/12673/12938/sbi%2520system%2520biosciences_1633075021__00205.original__03121.1633123705.jpg?c=1)
![Gene Knock-Out HR Targeting Vector [MCS1-EF1α-RFP-T2A-Puro-pA-MCS2] Gene Knock-Out HR Targeting Vector [MCS1-EF1α-RFP-T2A-Puro-pA-MCS2]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/12673/15643/Screenshot_2021-12-09_214631__77660.1640262373.png?c=1)
Gene Knock-Out HR Targeting Vector [MCS1-EF1α-RFP-T2A-Puro-pA-MCS2]
System Biosciences


Gene Knock-Out HR Targeting Vector with TK selection [MCS1-LoxP-EF1α-GFP-T2A-Puro-P2A-hsvTK-pA-LoxP-MCS2
System Biosciences
![GFP-T2A-Luciferase Co-Expression HR Targeting Vector [GFP-T2A-Luc-pA-loxP-EF1α-RFP-T2A-Puro-pA-LoxP-MCS] GFP-T2A-Luciferase Co-Expression HR Targeting Vector [GFP-T2A-Luc-pA-loxP-EF1α-RFP-T2A-Puro-pA-LoxP-MCS]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/12680/12945/sbi%2520system%2520biosciences_1633075021__00205.original__65000.1633123753.jpg?c=1)
![GFP-T2A-Luciferase Co-Expression HR Targeting Vector [GFP-T2A-Luc-pA-loxP-EF1α-RFP-T2A-Puro-pA-LoxP-MCS] GFP-T2A-Luciferase Co-Expression HR Targeting Vector [GFP-T2A-Luc-pA-loxP-EF1α-RFP-T2A-Puro-pA-LoxP-MCS]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/12680/15650/Screenshot_2021-12-09_224538__37530.1639071973.png?c=1)
GFP-T2A-Luciferase Co-Expression HR Targeting Vector [GFP-T2A-Luc-pA-loxP-EF1α-RFP-T2A-Puro-pA-LoxP-MCS]
System Biosciences


IRES-GFP Co-Expression HR Targeting Vector [IRES-GFP-pA-loxP-MCS1-EF1α-RFP-T2A-Puro-pA-LoxP-MCS2
System Biosciences
Customers Also Viewed


Super piggyBac Transposase expression vector (replaces PB200A-1), 50 rxns
System Biosciences


pMSCV-Human RORgt-EF1-GFP-T2A-Puro piggyBac transposon plasmid
System Biosciences


pMSCV-Mouse RORgt-EF1-GFP-T2A-Puro piggyBac transposon plasmid
System Biosciences


pMSCV-Human Foxp3-EF1-GFP-T2A-Puro piggyBac transposon plasmid
System Biosciences


pMSCV-Mouse Foxp3-EF1-GFP-T2A-Puro piggyBac transposon plasmid
System Biosciences


piggyBac qPCR copy number kit, 20 reactions
System Biosciences


PB-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector
System Biosciences


PB-SV40-Neo-EF1-GFP-CAGs-cMyc-Klf4-Oct4-Sox2 Mouse 4-in-1 iPSC Vector
System Biosciences


PB-EF1-MCS-IRES-Neo cDNA cloning and expression vector
System Biosciences


PB-Cuo-MCS-IRES-GFP-EF1-CymR-Puro Inducible cDNA Cloning and Expression Vector
System Biosciences


PB-CMV-MCS-EF1-Puro cDNA cloning and expression vector
System Biosciences


Excision only piggyBac Transposase expression vector, 50 rxns
System Biosciences


PB-MSCV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector
System Biosciences


PB-EF1-MCS-IRES-GFP cDNA cloning and expression vector
System Biosciences


PB-EF1-GreenPuro-H1-MCS shRNA cloning and expression vector
System Biosciences


PB-EF1a-Oct4-Sox2-Klf4-Myc-IRES-GFP Human 4-in-1 iPSC Vector
System Biosciences


PB-Cuo-shMCS-IRES-GFP-EF1-CymR-Puro Inducible shRNA Cloning and Expression Vector
System Biosciences


PB-CMV-MCS-EF1-GFP cDNA cloning and expression vector
System Biosciences


Cumate Solution, high concentration, 10,000x for use with PiggyBac
System Biosciences

PiggyBac Dual CMV-Pdgfb-m-mCherry (PB513B-1) Plasmid | PVT17112
Nova Lifetech

AAV Packaging Mix (Serotype 6) | AAV1006
ABM Good


XStamp Pro anti-HIV EV Targeting Kit
System Biosciences


XPack MSCV-XP-GFP-EF1-Puro Expression Lentivector
System Biosciences


Purified Exosomes from Human Urine (Healthy Donors)
System Biosciences


PB-CMV-MCS-EF1-RedPuro cDNA Cloning and Expression Vector
System Biosciences


PB-CMV-GreenPuro Scramble Hairpin Control Vector
System Biosciences


ExoQuick exosome precipitation solution (5 ml)
System Biosciences


AAVanced™ Concentration Reagent (250 ml aliquot)
System Biosciences

Super PiggyBac Transposase (PB200PA- 1) plasmid | PVT1602
Nova Lifetech

pCDNA3.1-SLC28A1-FLAG Plasmid | PVT12286
Nova Lifetech

pCDH-EF1-Luc-T2A-copGFP Plasmid | PVT17649
Nova Lifetech

EGR1-GFP (Bsd) Lentivirus | LVP1119-B
GenTarget

CD Lentiviral Vector | LV678
ABM Good

Lenti-III-CFP-N Complete Kit | LV670
ABM Good

Nucleus Labeling Lentivirus (LMNB1)(RFP) | LVP707
ABM Good

Nucleus Labeling Lentivirus (LMNB1)(GFP) | LVP706
ABM Good

Cytoskeleton Labeling Lentivirus (TUBA1B)(GFP) | LVP703
ABM Good

qPCR Lentivirus Complete Titration Kit (MasterMix-S) | LV900-S
ABM Good

AAV Packaging Mix (Serotype 5) & DNAfectin Combo Pack | AAV1005-G2100
ABM Good

2nd Gen. Packaging Mix & Lentifectin Combo Pack | LV003-G074
ABM Good

Lenti-EF1a-Blank Lentivirus | LVP688
ABM Good

ProAdhere 293T Cells | LV592
ABM Good

pLenti-CMV-GFP-2A-Puro-Blank Vector | LV590
ABM Good

Complete Lenti-His Kit | LV100
ABM Good

CD Lentiviral Virus | LV679
ABM Good

Adeno-2™ pShuttle-His Cloning Kit | A210
ABM Good

Adeno-1™ pShuttle(-) Cloning Kit | A120
ABM Good

Adeno-1™Kit | A110
ABM Good

Adeno-1™ Complete Cloning Kit | A100
ABM Good

Adeno Digest Kit with Cells | A098
ABM Good