System Biosciences
pMSCV-Mouse Foxp3-EF1-GFP-T2A-Puro piggyBac transposon plasmid
- SKU:
- TCL100PB-1
- UPC:
- MPN:
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 10ug
- Shipping Temperature:
- Blue Ice/ Dry Ice
- Qualifications:
- Requires signed license from commercial customers
Description
pMSCV-Mouse Foxp3-EF1-GFP-T2A-Puro piggyBac transposon plasmid. Cat# TCL100PB. Supplier: SBI System Biosciences
- Lentivector-based for transfection-resistant cell lines
- PiggyBac Transposon-based* for infection-resistant cell lines
- Pre-built Jurkat cell line to save time and labor
- Academic Customers—End-User License Information
- Commercial Customers—End-User License Information
Products

Overview
- Lentivector-based for transfection-resistant cell lines

- PiggyBac Transposon-based* for infection-resistant cell lines

Pre built Jurkat cell line to save time and labor
Quickly get your Treg studies off the ground with our pre-built Jurkat cell line that already has the pMSCV-Human RORC-EF1α-GFP-T2A-Puro expression cassette integrated into the genome. We transduced Jurkat cells with the pMSCV-Human RORC-EF1α-GFP-T2A-Puro expression cassette and have validated RORC overexpression, so you don’t have to—just culture cells and start your studies.
*Customer Agreements Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a six-month, limited-use license to test the technology.
For end user license information, see the following:
- Academic Customers—End-User License Information
- Commercial Customers—End-User License Information
* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Transposagen Biopharmaceuticals, Inc.
How It Works
A quick introduction to SBI’s lentivector packaging and PiggyBac Transposon technologies
Start producing lentivirus
The PiggyBac Transposon System’s Cut-and-Paste Mechanism
The efficient PiggyBac Transposon System uses a cut-and-paste mechanism to transfer DNA from the PiggyBac Vector into the genome. If only temporary genomic integration is desired, the Excision-only PiggyBac Transposase can be transiently expressed for footprint-free removal of the insert, resulting in reconstitution of the original genome sequence.
Figure 1. The PiggyBac Transposon System’s cut-and-paste mechanism.
The Super PiggyBac Transposase binds to specific inverted terminal repeats (ITRs) in the PiggyBac Cloning and Expression Vector and excises the ITRs and intervening DNA.
The Super PiggyBac Transposase inserts the ITR-Expression Cassette-ITR segment into the genome at TTAA sites.
The Excision-only Super PiggyBac Transposase can be used to remove the ITR-Expression Cassette-ITR segment from the genome, for footprint-free removal
Supporting Data
Robust overexpression of Foxp3
Figure 2. SBI’s human and mouse Foxp3 lentivector and PiggyBac plasmids provide robust Foxp3 overexpression. The human and mouse Foxp3 lentivectors Cat.# TCL200A-1 (human) and Cat.# TCL100A-1 (mouse) and the PiggyBac transposon constructs Cat.# TCL200PB-1 (human) and Cat.# TCL100PB-1 (mouse) were transiently transfected into 293FT cells. After 24 hours, cellular lysates were prepared and Western blot analyses performed to detect the Foxp3 protein expression using an anti-Foxp3 antibody that detects both human and mouse proteins. Robust overexpression of both human (lanes 1,3) and mouse (lanes 2,4) Foxp3 proteins compared to the non-transfected cell lysate control (lane 5) can be clearly observed, with tubulin as a loading control.
1 Review
-
5 Original
Posted by Maxwell on 10th Jan 2023
The product PMSCV-Mouse foxp3 was very effective and has very good quality and has no side effects given while using the product.
Related Products


pMSCV-Human Foxp3-EF1-GFP-T2A-Puro lentivector plasmid
System Biosciences


pMSCV-Human Foxp3-EF1-GFP-T2A-Puro piggyBac transposon plasmid
System Biosciences


pMSCV-Human RORgt-EF1-GFP-T2A-Puro piggyBac transposon plasmid
System Biosciences


pMSCV-Mouse Foxp3-EF1-GFP-T2A-Puro lentivector plasmid
System Biosciences


pMSCV-Mouse RORgt-EF1-GFP-T2A-Puro piggyBac transposon plasmid
System Biosciences
Customers Also Viewed


pMSCV-Human RORgt-EF1-GFP-T2A-Puro piggyBac transposon plasmid
System Biosciences


Super piggyBac Transposase expression vector (replaces PB200A-1), 50 rxns
System Biosciences


pMSCV-Mouse RORgt-EF1-GFP-T2A-Puro piggyBac transposon plasmid
System Biosciences


pMSCV-Human Foxp3-EF1-GFP-T2A-Puro piggyBac transposon plasmid
System Biosciences
![piggyBac-HR with GFP+Puro markers and TK selection [MCS1-5'PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3' PB TR-MCS2] for Gene Correctio piggyBac-HR with GFP+Puro markers and TK selection [MCS1-5'PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3' PB TR-MCS2] for Gene Correctio](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/14421/14689/sbi%20system%20biosciences_1633075021__00205.original__16600.1633076840.jpg?c=1)
![piggyBac-HR with GFP+Puro markers and TK selection [MCS1-5'PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3' PB TR-MCS2] for Gene Correctio piggyBac-HR with GFP+Puro markers and TK selection [MCS1-5'PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3' PB TR-MCS2] for Gene Correctio](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/14421/29947/PBHR100A-1__86845.1640081714.png?c=1)
piggyBac-HR with GFP+Puro markers and TK selection [MCS1-5'PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3' PB TR-MCS2] for Gene Correction
System Biosciences


piggyBac qPCR copy number kit, 20 reactions
System Biosciences


PB-SV40-Neo-EF1-GFP-CAGs-cMyc-Klf4-Oct4-Sox2 Mouse 4-in-1 iPSC Vector
System Biosciences


PB-MSCV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector
System Biosciences


PB-EF1-MCS-IRES-GFP cDNA cloning and expression vector
System Biosciences


PB-EF1a-Oct4-Sox2-Klf4-Myc-IRES-GFP Human 4-in-1 iPSC Vector
System Biosciences

Peroxisome Labeling Lentivirus (PTS)(CFP) | LVP726
ABM Good

ProAdhere 293T Cells | LV592
ABM Good

Adeno-1™ pShuttle(-) Cloning Kit | A120
ABM Good


PB-EF1-MCS-IRES-Neo cDNA cloning and expression vector
System Biosciences


PB-CMV-GreenPuro Scramble Hairpin Control Vector
System Biosciences

Human pre-microRNA Expression Construct Lenti-miR-544
System Biosciences

YCp50 | PVT11247
Nova Lifetech

Top- Flash | PVT1301
Nova Lifetech

Super PiggyBac Transposase (PB200PA- 1) plasmid | PVT1602
Nova Lifetech

PiggyBac Dual CMV-Pdgfb-m-mCherry (PB513B-1) Plasmid | PVT17112
Nova Lifetech

pEnCMV-wnt1-3 Plasmid | PVT17907
Nova Lifetech

pcDNA6-Flag-CBX4 Plasmid | PVTB00736-2a
Nova Lifetech

pCDNA4.0- TO/ Myc/ His B Plasmid | PVT1014
Nova Lifetech

ET12567 (pUZ8002) Escherichia coli Strains | S0052
Nova Lifetech

EGY48 Yeast Strains | S0112
Nova Lifetech

BJ5183/ pAdEasy-1 Escherichia coli Strains | S0125
Nova Lifetech

5'UTR CGG 99x FMR1-EGFP Plasmid | PVT18228
Nova Lifetech

EGR1-GFP (Bsd) Lentivirus | LVP1119-B
GenTarget

Autophagosome Labeling Lentivirus (MAP1LC3B)(GFP) | LVP730
ABM Good

Mitochondria Labeling Lentivirus (MTS)(RFP) | LVP722
ABM Good

Mitochondria Labeling Lentivirus (MTS)(GFP) | LVP721
ABM Good

pLenti-PGK-GFP Virus | LVP628
ABM Good

pLenti-PGK-Blank Virus | LVP627
ABM Good

AAV Packaging Mix (Serotype 4) & DNAfectin Combo Pack | AAV1004-G2100
ABM Good

AAV Packaging Mix (Serotype 2) | AAV1002
ABM Good

Adeno-8™ pShuttle-2A-RFP Complete Cloning Kit | A800
ABM Good

Add-N-Pure™ Adenovirus Purification Kit (5 U) | A905
ABM Good


SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)
System Biosciences

Rat SeraMir Exosome RNA 384 microRNA qPCR Profiler Assay Set
System Biosciences
![pLL-EF1a-Luciferase-T2A-Puro [Lenti-LabelerTM virus] pLL-EF1a-Luciferase-T2A-Puro [Lenti-LabelerTM virus]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/14469/14737/sbi%20system%20biosciences_1633075021__00205.original__06601.1633076847.jpg?c=1)
pLL-EF1a-Luciferase-T2A-Puro [Lenti-Labeler™ virus]
System Biosciences


PB-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector
System Biosciences


PB-EF1-GreenPuro-H1-MCS shRNA cloning and expression vector
System Biosciences


PB-Cuo-shMCS-IRES-GFP-EF1-CymR-Puro Inducible shRNA Cloning and Expression Vector
System Biosciences


PB-CMV-MCS-EF1-Puro cDNA cloning and expression vector
System Biosciences


PB-CMV-MCS-EF1-GFP cDNA cloning and expression vector
System Biosciences

Mouse pre-microRNA Expression Construct mir-92a-2
System Biosciences

miRZip-199b-3p anti-miR-199b-3p microRNA construct
System Biosciences

miRZip-184 anti-miR-184 microRNA construct
System Biosciences


Exosome-Depleted FBS Media Supplement Heat Inactivated
System Biosciences


ExoQuick TC 50 ml
System Biosciences