System Biosciences
pMSCV-Human Foxp3-EF1-GFP-T2A-Puro piggyBac transposon plasmid
- SKU:
- TCL200PB-1
- UPC:
- MPN:
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 10ug
- Shipping Temperature:
- Blue Ice/ Dry Ice
- Qualifications:
- Requires signed license from commercial customers
Description
pMSCV-Human Foxp3-EF1-GFP-T2A-Puro piggyBac transposon plasmid. Cat# TCL200PB. Supplier: SBI System Biosciences
- Academic Customers—End-User License Information
- Commercial Customers—End-User License Information
Products

Overview
Study the balance of T cell dynamics in autoimmunity, inflammation, and cancer
Simplify your studies of T cell dynamics with SBI’s ready-to-use, pre-built Treg and Th17 vectors and cell lines. With pMSCV-Human Foxp3-EF1α-GFP-T2A-Puro for Foxp3 Overexpression, you can choose between three different ways to study Foxp3 overexpression from the MSCV promoter for strong expression in hematopoietic and stem cells:
Lentivector-based for transfection-resistant cell lines
Create stable Foxp3-overexpressing cell lines by integrating the MSCV-Human Foxp3-EF1α-GFP-T2A-Puro expression cassette into the genome using SBI’s highly regarded and reliable lentivector technology. With our lentivector technology, you can take advantage of our high-titer lentivector packaging system to efficiently deliver your Foxp3 overexpression cassette to cells using transduction—great for transfection-resistant cell lines.
PiggyBac Transposon-based* for infection-resistant cell lines
Create stable Foxp3-overexpressing cell lines by integrating the MSCV-Human Foxp3-EF1α-GFP-T2A-Puro expression cassette into the genome with SBI’s easy and consistent PiggyBac Transposon System. With our PiggyBac Transposon System, you get easy and consistent transgenesis for Foxp3 overexpression in infection-resistant cell lines.
Pre-built Jurkat cell line to save time and labor
Quickly get your Treg studies off the ground with our pre-built Jurkat cell line that already has the MSCV-Human Foxp3-EF1α-GFP-T2A-Puro expression cassette integrated into the genome. We transduced Jurkat cells with the MSCV-Human Foxp3-EF1α-GFP-T2A-Puro expression cassette and have validated Foxp3 overexpression, so you don’t have to—just culture cells and start your studies.
*Customer Agreements
Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a six-month, limited-use license to test the technology. For end user license information, see the following:
- Academic Customers—End-User License Information
- Commercial Customers—End-User License Information
* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Transposagen Biopharmaceuticals, Inc.
How It Works
A quick introduction to SBI’s lentivector packaging and PiggyBac Transposon technologies
Start producing lentivirus
The PiggyBac Transposon System’s Cut-and-Paste Mechanism
The efficient PiggyBac Transposon System uses a cut-and-paste mechanism to transfer DNA from the PiggyBac Vector into the genome. If only temporary genomic integration is desired, the Excision-only PiggyBac Transposase can be transiently expressed for footprint-free removal of the insert, resulting in reconstitution of the original genome sequence.
Figure 1. The PiggyBac Transposon System’s cut-and-paste mechanism.
1. The Super PiggyBac Transposase binds to specific inverted terminal repeats (ITRs) in the PiggyBac Cloning and Expression Vector and excises the ITRs and intervening DNA.
2. The Super PiggyBac Transposase inserts the ITR-Expression Cassette-ITR segment into the genome at TTAA sites.
3. The Excision-only Super PiggyBac Transposase can be used to remove the ITR-Expression Cassette-ITR segment from the genome, for footprint-free removal
Supporting Data
Robust overexpression of Foxp3
Figure 2. SBI’s human and mouse Foxp3 lentivector and PiggyBac plasmids provide robust Foxp3 overexpression. The human and mouse Foxp3 lentivectors Cat.# TCL200A-1 (human) and Cat.# TCL100A-1 (mouse) and the PiggyBac transposon constructs Cat.# TCL200PB-1 (human) and Cat.# TCL100PB-1 (mouse) were transiently transfected into 293FT cells. After 24 hours, cellular lysates were prepared and Western blot analyses performed to detect the Foxp3 protein expression using an anti-Foxp3 antibody that detects both human and mouse proteins. Robust overexpression of both human (lanes 1,3) and mouse (lanes 2,4) Foxp3 proteins compared to the non-transfected cell lysate control (lane 5) can be clearly observed, with tubulin as a loading control.
Figure 3. SBI’s MSCV-Human Foxp3-EF1α-GFP-T2A-Puro Jurkat Cell Line shows strong Foxp3 overexpression. Human Jurkat T cells were transduced with either Cat.# TCL200A-1 (human) or Cat.# TCL100A-1 (mouse) pre-packaged lentivirus and stable cell lines established using puromycin selection for seven days. Overexpression of Foxp3 was evaluated by measuring GFP levels using flow cytometry and Western blot analysis of cellular lysates and using anti-Foxp3 antibody. Both methods show that SBI’s Foxp3-overexpressing Jurkat Cell Line possesses strong Foxp3 overexpression.
1 Review
-
5 Excellent
Posted by William on 10th Jan 2023
The human FOXP3 EF1 GFP T2A puro piggbac was very excellent product and I am very satisfied with it. Will reorder soon.
Related Products


pMSCV-Human Foxp3-EF1-GFP-T2A-Puro lentivector plasmid
System Biosciences


pMSCV-Human RORgt-EF1-GFP-T2A-Puro piggyBac transposon plasmid
System Biosciences


pMSCV-Mouse Foxp3-EF1-GFP-T2A-Puro lentivector plasmid
System Biosciences


pMSCV-Mouse Foxp3-EF1-GFP-T2A-Puro piggyBac transposon plasmid
System Biosciences


pMSCV-Mouse RORgt-EF1-GFP-T2A-Puro piggyBac transposon plasmid
System Biosciences
Customers Also Viewed


pMSCV-Human RORgt-EF1-GFP-T2A-Puro piggyBac transposon plasmid
System Biosciences


pMSCV-Mouse RORgt-EF1-GFP-T2A-Puro piggyBac transposon plasmid
System Biosciences


pMSCV-Mouse Foxp3-EF1-GFP-T2A-Puro piggyBac transposon plasmid
System Biosciences
![piggyBac-HR with GFP+Puro markers and TK selection [MCS1-5'PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3' PB TR-MCS2] for Gene Correctio piggyBac-HR with GFP+Puro markers and TK selection [MCS1-5'PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3' PB TR-MCS2] for Gene Correctio](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/14421/14689/sbi%20system%20biosciences_1633075021__00205.original__16600.1633076840.jpg?c=1)
![piggyBac-HR with GFP+Puro markers and TK selection [MCS1-5'PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3' PB TR-MCS2] for Gene Correctio piggyBac-HR with GFP+Puro markers and TK selection [MCS1-5'PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3' PB TR-MCS2] for Gene Correctio](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/14421/29947/PBHR100A-1__86845.1640081714.png?c=1)
piggyBac-HR with GFP+Puro markers and TK selection [MCS1-5'PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3' PB TR-MCS2] for Gene Correction
System Biosciences


Super piggyBac Transposase expression vector (replaces PB200A-1), 50 rxns
System Biosciences


PB-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector
System Biosciences


PB-EF1-MCS-IRES-Neo cDNA cloning and expression vector
System Biosciences


piggyBac qPCR copy number kit, 20 reactions
System Biosciences


PB-Cuo-MCS-IRES-GFP-EF1-CymR-Puro Inducible cDNA Cloning and Expression Vector
System Biosciences


PB-SV40-Neo-EF1-GFP-CAGs-cMyc-Klf4-Oct4-Sox2 Mouse 4-in-1 iPSC Vector
System Biosciences


PB-MSCV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector
System Biosciences


PB-EF1-MCS-IRES-GFP cDNA cloning and expression vector
System Biosciences


PB-EF1-GreenPuro-H1-MCS shRNA cloning and expression vector
System Biosciences


PB-CMV-MCS-EF1-Puro cDNA cloning and expression vector
System Biosciences


PB-EF1a-Oct4-Sox2-Klf4-Myc-IRES-GFP Human 4-in-1 iPSC Vector
System Biosciences


PB-Cuo-shMCS-IRES-GFP-EF1-CymR-Puro Inducible shRNA Cloning and Expression Vector
System Biosciences


PB-CMV-GreenPuro Scramble Hairpin Control Vector
System Biosciences


Excision only piggyBac Transposase expression vector, 50 rxns
System Biosciences

Super PiggyBac Transposase (PB200PA- 1) plasmid | PVT1602
Nova Lifetech

PiggyBac Dual CMV-Pdgfb-m-mCherry (PB513B-1) Plasmid | PVT17112
Nova Lifetech

Anti-Mesothelin (SS1) CAR4 Lentivirus | LVP754
ABM Good

qPCR Lentivirus Complete Titration Kit (MasterMix-R) | LV900-R
ABM Good

pLenti-PGK-Blank Virus | LVP627
ABM Good

AAV Packaging Mix (Serotype 4) & DNAfectin Combo Pack | AAV1004-G2100
ABM Good

AAV Packaging Mix (Serotype 4) | AAV1004
ABM Good

AAV Packaging Mix (Serotype 2) & DNAfectin Combo Pack | AAV1002-G2100
ABM Good

AAV Packaging Mix (Serotype 2) | AAV1002
ABM Good

3rd Gen. Packaging Mix & Lentifectin Combo Pack | LV053-G074
ABM Good

Lenti-UbC-Blank Lentivirus | LVP689
ABM Good

Adeno-8™ pShuttle-2A-RFP Complete Cloning Kit | A800
ABM Good

Adeno-2™ pShuttle-His-18x Complete Cloning Kit | A240
ABM Good

Complete Lenti-His Kit | LV100
ABM Good


XStamp IL2 exosome IL-2 receptor targeting lentivector
System Biosciences


SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)
System Biosciences

Rat SeraMir Exosome RNA 384 microRNA qPCR Profiler Assay Set
System Biosciences


PureFection™ Transfection reagent
System Biosciences


PureFection Transfection Reagent
System Biosciences
![pLL-EF1a-RFP-T2A-Blast [Lenti-LabelerTM virus] pLL-EF1a-RFP-T2A-Blast [Lenti-LabelerTM virus]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/14475/14743/sbi%20system%20biosciences_1633075021__00205.original__20553.1633076848.jpg?c=1)
![pLL-EF1a-RFP-T2A-Blast [Lenti-LabelerTM virus] pLL-EF1a-RFP-T2A-Blast [Lenti-LabelerTM virus]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/14475/30001/LL215VA__02932.1640248818.png?c=1)
pLL-EF1a-RFP-T2A-Blast [Lenti-Labeler™ virus]
System Biosciences
![pLL-EF1a-Luciferase-T2A-Puro [Lenti-LabelerTM virus] pLL-EF1a-Luciferase-T2A-Puro [Lenti-LabelerTM virus]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/14469/14737/sbi%20system%20biosciences_1633075021__00205.original__06601.1633076847.jpg?c=1)
pLL-EF1a-Luciferase-T2A-Puro [Lenti-Labeler™ virus]
System Biosciences


PB-CMV-MCS-EF1-GreenPuro cDNA cloning and expression vector
System Biosciences

miRZip-184 anti-miR-184 microRNA construct
System Biosciences


Exosome-Depleted FBS Media Supplement
System Biosciences


ExoQuick TC 50 ml
System Biosciences


ExoQuick Exosome RNA Column Purification kit
System Biosciences


Cumate Solution, high concentration, 10,000x for use with PiggyBac
System Biosciences


Cas9 Nickase: CAG-hspCas9-nickase-H1-gRNA SmartNickase vector
System Biosciences
![Basic HR Targeting Vector [MCS1-LoxP-MCS2-MCS3-pA-LoxP-MCS4] for Gene Knock-In/Out Basic HR Targeting Vector [MCS1-LoxP-MCS2-MCS3-pA-LoxP-MCS4] for Gene Knock-In/Out](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/12477/12741/sbi%20system%20biosciences_1633075021__00205.original__94784.1633076533.jpg?c=1)
![Basic HR Targeting Vector [MCS1-LoxP-MCS2-MCS3-pA-LoxP-MCS4] for Gene Knock-In/Out Basic HR Targeting Vector [MCS1-LoxP-MCS2-MCS3-pA-LoxP-MCS4] for Gene Knock-In/Out](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/12477/15488/HR100PA-1__03435.1638779819.jpg?c=1)
Basic HR Targeting Vector [MCS1-LoxP-MCS2-MCS3-pA-LoxP-MCS4] for Gene Knock-In/Out
System Biosciences


AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS)
System Biosciences


AAVanced™ Concentration Reagent (100 ml aliquot)
System Biosciences

5'UTR CGG 99x FMR1-EGFP Plasmid | PVT18228
Nova Lifetech