System Biosciences
pMSCV-Human RORgt-EF1-GFP-T2A-Puro piggyBac transposon plasmid
- SKU:
- TCL400PB-1
- UPC:
- MPN:
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 10ug
- Shipping Temperature:
- Blue Ice/ Dry Ice
- Qualifications:
- Requires signed license from commercial customers
Description
pMSCV-Human RORgt-EF1-GFP-T2A-Puro piggyBac transposon plasmid. Cat# TCL400PB. Supplier: SBI System Biosciences
- Academic Customers—End-User License Information
- Commercial Customers—End-User License Information
Products

Overview
Study the balance of T cell dynamics in autoimmunity, inflammation, and cancer
Simplify your studies of T cell dynamics with SBI’s ready-to-use, pre-built Treg and Th17 vectors and cell lines. With pMSCV-Human RORC-EF1α-GFP-T2A-Puro for RORC Overexpression, you can choose between three different ways to study RORC overexpression from the MSCV promoter for strong expression in hematopoietic and stem cells:
Lentivector-based for transfection-resistant cell lines
Create stable RORC-overexpressing cell lines by integrating the pMSCV-Human RORC-EF1α-GFP-T2A-Puro expression cassette into the genome using SBI’s highly regarded and reliable lentivector technology. With our lentivector technology, you can take advantage of our high-titer lentivector packaging system to efficiently deliver your RORC overexpression cassette to cells using transduction—great for transfection-resistant cell lines.
PiggyBac Transposon-based* for infection-resistant cell lines
Create stable RORC-overexpressing cell lines by integrating the pMSCV-Human RORC-EF1α-GFP-T2A-Puro expression cassette into the genome with SBI’s easy and consistent PiggyBac Transposon System. With our PiggyBac Transposon System, you get easy and consistent transgenesis for RORC overexpression in infection-resistant cell lines.
Pre built Jurkat cell line to save time and labor
Quickly get your Treg studies off the ground with our pre-built Jurkat cell line that already has the pMSCV-Human RORC-EF1α-GFP-T2A-Puro expression cassette integrated into the genome. We transduced Jurkat cells with the pMSCV-Human RORC-EF1α-GFP-T2A-Puro expression cassette and have validated RORC overexpression, so you don’t have to—just culture cells and start your studies.
*Customer Agreements Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a six-month, limited-use license to test the technology.
For end user license information, see the following:
- Academic Customers—End-User License Information
- Commercial Customers—End-User License Information
* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Transposagen Biopharmaceuticals, Inc.
How It Works
A quick introduction to SBI’s lentivector packaging and PiggyBac Transposon technologies
Start producing lentivirus
Figure 1. The PiggyBac Transposon System’s cut-and-paste mechanism.
1. The Super PiggyBac Transposase binds to specific inverted terminal repeats (ITRs) in the PiggyBac Cloning and Expression Vector and excises the ITRs and intervening DNA.
2. The Super PiggyBac Transposase inserts the ITR-Expression Cassette-ITR segment into the genome at TTAA sites.
3. The Excision-only Super PiggyBac Transposase can be used to remove the ITR-Expression Cassette-ITR segment from the genome, for footprint-free removal
Supporting Data
Robust overexpression of RORC
1 Review
-
4 perfect
Posted by Elliot on 10th Jan 2023
The product I ordered from repotergene.com was perfect in giving results and was highly effective.
Related Products


pMSCV-Human Foxp3-EF1-GFP-T2A-Puro piggyBac transposon plasmid
System Biosciences


pMSCV-Human RORgt-EF1-GFP-T2A-Puro lentivector plasmid
System Biosciences


pMSCV-Mouse Foxp3-EF1-GFP-T2A-Puro piggyBac transposon plasmid
System Biosciences


pMSCV-Mouse RORgt-EF1-GFP-T2A-Puro lentivector plasmid
System Biosciences


pMSCV-Mouse RORgt-EF1-GFP-T2A-Puro piggyBac transposon plasmid
System Biosciences
Customers Also Viewed


pMSCV-Mouse Foxp3-EF1-GFP-T2A-Puro piggyBac transposon plasmid
System Biosciences


pMSCV-Mouse RORgt-EF1-GFP-T2A-Puro piggyBac transposon plasmid
System Biosciences


pMSCV-Human Foxp3-EF1-GFP-T2A-Puro piggyBac transposon plasmid
System Biosciences


Super piggyBac Transposase expression vector (replaces PB200A-1), 50 rxns
System Biosciences
![piggyBac-HR with GFP+Puro markers and TK selection [MCS1-5'PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3' PB TR-MCS2] for Gene Correctio piggyBac-HR with GFP+Puro markers and TK selection [MCS1-5'PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3' PB TR-MCS2] for Gene Correctio](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/14421/14689/sbi%20system%20biosciences_1633075021__00205.original__16600.1633076840.jpg?c=1)
![piggyBac-HR with GFP+Puro markers and TK selection [MCS1-5'PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3' PB TR-MCS2] for Gene Correctio piggyBac-HR with GFP+Puro markers and TK selection [MCS1-5'PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3' PB TR-MCS2] for Gene Correctio](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/14421/29947/PBHR100A-1__86845.1640081714.png?c=1)
piggyBac-HR with GFP+Puro markers and TK selection [MCS1-5'PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3' PB TR-MCS2] for Gene Correction
System Biosciences


PB-EF1-MCS-IRES-Neo cDNA cloning and expression vector
System Biosciences


PB-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector
System Biosciences


PB-CMV-MCS-EF1-Puro cDNA cloning and expression vector
System Biosciences


PB-MSCV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector
System Biosciences


PB-EF1-MCS-IRES-GFP cDNA cloning and expression vector
System Biosciences


piggyBac qPCR copy number kit, 20 reactions
System Biosciences


PB-SV40-Neo-EF1-GFP-CAGs-cMyc-Klf4-Oct4-Sox2 Mouse 4-in-1 iPSC Vector
System Biosciences


Cumate Solution, high concentration, 10,000x for use with PiggyBac
System Biosciences

Super PiggyBac Transposase (PB200PA- 1) plasmid | PVT1602
Nova Lifetech


PB-EF1-GreenPuro-H1-MCS shRNA cloning and expression vector
System Biosciences


PB-EF1a-Oct4-Sox2-Klf4-Myc-IRES-GFP Human 4-in-1 iPSC Vector
System Biosciences


PB-Cuo-MCS-IRES-GFP-EF1-CymR-Puro Inducible cDNA Cloning and Expression Vector
System Biosciences


Excision only piggyBac Transposase expression vector, 50 rxns
System Biosciences

Rat SeraMir Exosome RNA 384 microRNA qPCR Profiler Assay Set
System Biosciences
![pLL-EF1a-Luciferase-T2A-Puro [Lenti-LabelerTM virus] pLL-EF1a-Luciferase-T2A-Puro [Lenti-LabelerTM virus]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/14469/14737/sbi%20system%20biosciences_1633075021__00205.original__06601.1633076847.jpg?c=1)
pLL-EF1a-Luciferase-T2A-Puro [Lenti-Labeler™ virus]
System Biosciences


PB-Cuo-shMCS-IRES-GFP-EF1-CymR-Puro Inducible shRNA Cloning and Expression Vector
System Biosciences


PB-CMV-MCS-EF1-RedPuro cDNA Cloning and Expression Vector
System Biosciences


PB-CMV-MCS-EF1-GreenPuro cDNA cloning and expression vector
System Biosciences


PB-CMV-GreenPuro Scramble Hairpin Control Vector
System Biosciences


ExoQuick ULTRA EV Isolation Kit for Serum and Plasma
System Biosciences

PiggyBac Dual CMV-Pdgfb-m-mCherry (PB513B-1) Plasmid | PVT17112
Nova Lifetech

Mitochondria Labeling Lentivirus (MTS)(GFP) | LVP721
ABM Good

Complete Lenti-His Kit | LV100
ABM Good

Adeno-4™ pSiShuttle Cloning Kit | A410
ABM Good


XStamp MLN exosome Motilin receptor GI tract targeting lentivector
System Biosciences


XCF COMPLETE Exosome and cfDNA Isolation Kit (for Serum and Plasma)
System Biosciences


PureFection Transfection Reagent
System Biosciences
![pLL-EF1a-RFP-T2A-Blast [Lenti-LabelerTM virus] pLL-EF1a-RFP-T2A-Blast [Lenti-LabelerTM virus]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/14475/14743/sbi%20system%20biosciences_1633075021__00205.original__20553.1633076848.jpg?c=1)
![pLL-EF1a-RFP-T2A-Blast [Lenti-LabelerTM virus] pLL-EF1a-RFP-T2A-Blast [Lenti-LabelerTM virus]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/14475/30001/LL215VA__02932.1640248818.png?c=1)
pLL-EF1a-RFP-T2A-Blast [Lenti-Labeler™ virus]
System Biosciences

miRZip-184 anti-miR-184 microRNA construct
System Biosciences


ExoQuick exosome precipitation solution (20 ml)
System Biosciences


Exo-FITC Exosome FACS stain
System Biosciences


Cas9 Nickase: CAG-T7-hspCas9-nickase-T2A-GFP-H1-gRNA linearized SmartNickase vector
System Biosciences


Anti-CETP Antibody (polyclonal rabbit anti-human, mouse, rat) with goat anti-rabbit HRP secondary antibody
System Biosciences

XL10-Gold Escherichia coli Strains | S0029
Nova Lifetech

Top10 Escherichia coli Strains | S0003
Nova Lifetech

pCDNA3.1-SLC28A1-FLAG Plasmid | PVT12286
Nova Lifetech

cgi-EGFP-HERG(Y652A) Plasmid | PVTB01024-2d
Nova Lifetech

5'UTR CGG 99x FMR1-EGFP Plasmid | PVT18228
Nova Lifetech

1136- Desmoplakin- GFP | PVT10351
Nova Lifetech

EGR1-GFP (Bsd) Lentivirus | LVP1119-B
GenTarget

ZYX Clone BC008743
Cusabio

Anti-Mesothelin (SS1) CAR4 Lentivirus | LVP754
ABM Good

Lenti-III-CFP-C Partial Kit | LV673
ABM Good

Mitochondria Labeling Lentivirus (MTS)(CFP) | LVP723
ABM Good

Mitochondria Labeling Lentivirus (MTS)(RFP) | LVP722
ABM Good