System Biosciences
SmartSEC Single EV Isolation System
- SKU:
- SSEC200A-1
- UPC:
- MPN:
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 10 rxn
- Shipping Temperature:
- RT or Blue Ice
Description
SmartSEC Single EV Isolation System. Cat# SSEC200A. Supplier: SBI System Biosciences
- Quick and easy isolation
- Better purity and yield than ultracentrifugation
- Higher EV concentration per fraction than competitors’ conventional size exclusion chromatography kits
- Validated for human serum, plasma, and CSF as well as Aplysia californica hemolymph
- Compatible with most downstream applications such as mass spectrometry, western blotting, nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM) and RNA-sequencing (NGS)
Products

Overview
Get outstanding SEC-based EV isolation with SmartSEC™ Single
SmartSEC Single uses the same proprietary chromatography-based extracellular vesicle (EV) isolation technology as our popular SmartSEC HT plate and SmartSEC Mini kit but in a single tube format. SmartSEC technology combines all the benefits of size exclusion chromatography (SEC)—purity, yield, reproducibility, and preservation of EV integrity—with a contaminant trapping feature that enhances the capabilities of conventional SEC. The result is best-in-class EV isolation that is fast, easy, and clean.- Quick and easy isolation
- Better purity and yield than ultracentrifugation
- Higher EV concentration per fraction than competitors’ conventional size exclusion chromatography kits
- Validated for human serum, plasma, and CSF as well as Aplysia californica hemolymph
- Compatible with most downstream applications such as mass spectrometry, western blotting, nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM) and RNA-sequencing (NGS)
How It Works

Supporting Data
SmartSEC Single performs better than competitor’s q SEC technology
To understand how well SmartSEC Single performs compared to a competitor’s q SEC columns, we isolated EVs from 250 µL of human serum using both SmartSEC Single and q SEC columns and analyzed 1 µg of protein equivalent from each isolation method on a western blot (Figure 1) and using fluorescent nanoparticle tracking analysis (fNTA, Figure 2).

SmartSEC Single cleanly isolates EVs from CSF
SmartSEC Single can isolate EVs from cerebrospinal fluid (CSF) with no detectable cellular contamination, as shown in the ExoCheck Neuro Array in Figure 3. The isolated EVs possess both neuronal and exosomal markers, suggesting that the EV prep is enriched for vesicles of neuronal origin. Interestingly, EVs isolated from CSF tend to be smaller in size than EVs isolated from serum (Figure 4).

EVs isolated using Smart
(TEM) of EVs isolated from serum using SmartSEC Single possess typical EV morphology—intact vesicles with a double layer of membranes—with little visible background debris (Figure 5).
EVs isolated with SmartSEC Single and a competitor’s q SEC columns have very similar proteomic profiles.
We analyzed EVs isolated from human serum using SmartSEC Single and a competitor’s q SEC columns using mass spectrometry. Both methods yielded very similar proteomes, with 86% overlap (Figure 6).
SmartSEC Single can be used to isolate EVs for downstream proteomic studies.
To demonstrate the versatility and power of SmartSEC Single for enabling EV research, we used SmartSEC Single technology to isolate EVs from normal serum and serum from prostate cancer patients and used mass spectrometry to analyze the respective proteomes. A selection of identified proteins is shown in the table below, and the full list can be downloaded from here.
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