System Biosciences
XPack CMV-XP-GFP-EF1-Puro Stable HEK293 Producer Cell Line
- SKU:
- XPAK530CL-1
- UPC:
- MPN:
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 2 x 10^6 cells
- Shipping Temperature:
- Dry Ice
Description
XPack CMV-XP-GFP-EF1-Puro Stable HEK293 Producer Cell Line. Cat# XPAK530CL. Supplier: SBI System Biosciences
- Powerful—enables a wealth of applications, including Exosome labeling
Exosome cargo tracking
Exosomes as cargo delivery vehicles
Exosomes as biotherapeutics
Exosome-generating stable cell lines with protein cargo-of-interest - Exosome labeling
- Exosome cargo tracking
- Exosomes as cargo delivery vehicles
- Exosomes as biotherapeutics
Products
Overview
- Powerful—enables a wealth of applications, including
- Exosome labeling
- Exosome cargo tracking
- Exosomes as cargo delivery vehicles
- Exosomes as biotherapeutics
- Exosome-generating stable cell lines with protein cargo-of-interest
- Optimized—SBI developed the XPack peptide tag for robust exosome targeting
- Reliable—based on SBI’s highly-cited, well-proven lentivector technology
- Versatile
- Two promoter options for expression in a wide range of cells
- Comes as pre-built reporters (GFP, RFP, luciferase) or ready-to-customize cloning lentivectors
Choose the XPack Lentivector that’s right for you
How It Works
Optimized for success
With XPack, SBI has developed an optimized N-terminal peptide sequence that targets the peptide—and any protein fused to it—to the inner surface of the exosome membrane. Our pre-built XPack-reporter constructs come in GFP, RFP, and luciferase options to cover a wide range of labeling needs.
Supporting Data
XPack efficiently packages GFP into exosomes which can be used to deliver GFP to target cells
Part 1: Packaging GFP into exosomes with XPack
The XPack-GFP fusion protein is efficiently packaged as exosome cargo and delivered to target cells. HEK-293 cells were transfected with a plasmid encoding an N-terminal XPack-GFP fusion protein (Cat.# XPAK530PA-1). Twenty-four hours later, GFP localization patterns were visualized on a Leica DMI300B fluorescent microscope (Figure 1, upper panel). GFP expression patterns within transfected cells demonstrate a localization pattern characteristic of exosomal loading, with punctate spots along the cell periphery indicating budding vesicles and a brightly fluorescent spot in the cell interior corresponding to the multivesicular body (MVB), the site of exosome assembly and maturation prior to secretion.
After 48 hours, exosomes were isolated using ExoQuick-TC® and analyzed for GFP via Western blot (Figure 1, lower panel). Equal amounts of exosomal lysates (15 µg) were separated on gradient SDS PAGE gels and detected for GFP protein expression. The control for exosome loading was an anti-CETP antibody. XPack-GFP exosomes had very high amounts of GFP protein loaded as cargo.
Figure 1. XPack efficiently packages GFP into exosomes. (Upper panel) XPack-GFP localization during packaging. (Lower panel) Western blot showing robust loading of GFP into the isolated exosomes.
Part 2: XPack-GFP Exosome Delivery to Target Cells
The XPack-GFP exosomes isolated as described above were then added to recipient HEK-293 cells to test for GFP delivery. Cells exposed to XPack-GFP exosomes were imaged by fluorescent microscopy, which showed subcellular GFP localization consistent with endocytosis of the XPack-GFP exosomes and delivery of the XPack-GFP protein cargo into the cell (Figure 2, left panels). Subsequently, these recipient cells were washed twice with PBS and cellular lysates were prepared for Western blot analysis using anti-GFP antibodies. Anti-GAPDH was used as a cellular protein loading control. The Western blot data further verified the successful delivery of GFP proteins to recipient cells via the XPack exosome engineering technology (Figure 2, right panel).
Figure 2. XPack-GFP exosomes deliver GFP to target cells. (Left panel) XPack-GFP localization during packaging. (Right panel) Western blot showing robust GFP delivery to target cells.
Related Products


XPack CMV-XP-GFP-EF1-Puro Expression Lentivector
System Biosciences


XPack CMV-XP-GFP-EF1-Puro Expression Lentivirus
System Biosciences


XPack CMV-XP-Luciferase-EF1-Puro Expression Lentivirus
System Biosciences


XPack CMV-XP-Luciferase-EF1-PuroStable HEK293 Producer Cell Line
System Biosciences


XPack CMV-XP-RFP-EF1-Puro Expression Lentivector
System Biosciences
Customers Also Viewed

ZZZ3 Clone BC035818
Cusabio

Plasma Membrane Labeling Lentivirus (LCK)(RFP) | LVP713
ABMGood

Plasma Membrane Labeling Lentivirus (LCK)(GFP) | LVP712
ABMGood

AAV Serotype Blast™ Kit | AAV099
ABMGood

Anti-Mesothelin (SS1) TCAR Lentivirus | LVP750
ABMGood

Adeno-1™ Complete Cloning Kit | A100
ABMGood


XStamp Pro Streptavidin Customizable EV Targeting Kit
System Biosciences


XStamp Pro anti-PD-1 EV Targeting Kit
System Biosciences


XStamp MLN exosome Motilin receptor GI tract targeting lentivector
System Biosciences


XStamp BHP1 exosome brain homing peptide targeting lentivector
System Biosciences


XPack MSCV-XP-Luciferase-EF1-Puro Expression Lentivirus
System Biosciences


XPack MSCV-XP-GFP-EF1-Puro Expression Lentivector
System Biosciences


XCF COMPLETE Exosome and cfDNA Isolation Kit (for Serum and Plasma)
System Biosciences


SmartSEC™ HT EV Isolation System (for Serum & Plasma)
System Biosciences


SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns)
System Biosciences


SeraMir Exosome RNA Purification kit (10ml ExoQuick-TC and 10 exoRNA columns) 10 preps
System Biosciences


SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)
System Biosciences


Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800TC-1 with 50ml ExoQuick-TC, RA805A-1 and RA812A-1 components)
System Biosciences


PCS-500-012 Human bone marrow-derived mesenchymal stem cell line: >1x10^10 frozen exosomes
System Biosciences


PCS-500-011 Human pre-adipose derived mesenchymal stem cell: >1x10^10 frozen exosomes
System Biosciences

miRZip-199a-3p anti-miR-199a-3p microRNA construct
System Biosciences

miRZip-188-3p anti-miR-188-3p microRNA construct
System Biosciences

miRZip-1248 anti-miR-1248 microRNA construct bacterial streak
System Biosciences


Human Exosome lysate positive protein control (10ug/ul), Size 50 ul
System Biosciences


FluoroCet Exosome Quantitation Kit
System Biosciences


Exo-Urine EV Isolation Kit
System Biosciences


Exosome-Depleted FBS Media Supplement Heat Inactivated
System Biosciences


Exosome ELISA Complete Kit (CD9 detection) 96 reactions
System Biosciences


Exosome ELISA Complete Kit (CD63 detection) 96 rxns
System Biosciences


Exosome binding buffer for ELISAs (20 ml)
System Biosciences


ExoQuick-TC PLUS Exosome Purification Kit - for tissue culture media
System Biosciences


ExoQuick-LP for lipoprotein pre-clear & exosome isolation
System Biosciences


ExoQuick-CG cGMP-grade Exosome Isolation reagent (50 ml)
System Biosciences


ExoQuick ULTRA EV Isolation Kit for Serum and Plasma
System Biosciences


ExoQuick TC 50 ml
System Biosciences


ExoQuick TC 10 ml
System Biosciences


ExoQuick PLUS Exosome Purification Kit - for serum and plasma
System Biosciences


ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL)
System Biosciences


ExoQuick Exosome RNA Column Purification kit
System Biosciences


ExoQuick exosome precipitation solution (5 ml)
System Biosciences


ExoELISA-ULTRA Complete Kit (CD63 detection)
System Biosciences
![Cold Fusion Cloning Kit [96X] with 96-well format for Competent Cells -- International Sales Only Cold Fusion Cloning Kit [96X] with 96-well format for Competent Cells -- International Sales Only](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/12550/12814/sbi%20system%20biosciences_1633075021__00205.original__77334.1633076544.jpg?c=1)
![Cold Fusion Cloning Kit [96X] with 96-well format for Competent Cells -- International Sales Only Cold Fusion Cloning Kit [96X] with 96-well format for Competent Cells -- International Sales Only](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/12550/15557/Screenshot_2021-12-07_184716__84875.1638884864.png?c=1)
Cold Fusion Cloning Kit [96X] with 96-well format for Competent Cells -- International Sales Only
System Biosciences


Cas9 Nickase: CAG-T7-hspCas9-nickase-T2A-GFP-H1-gRNA linearized SmartNickase vector
System Biosciences


96 Well ExoELISA plate (12x8 well strips, pack of 10 plates)
System Biosciences


293TN Producer Cell Line
System Biosciences

SGLT1 Plasmid | PVT7136
Nova Lifetech

pEnCMV-PGAM5-3 Plasmid | PVT18363
Nova Lifetech

pcDNA6/myc-HisA-WWP1 Plasmid | PVTB00443-2a
Nova Lifetech

pCDNA3-PTGER2-EGFP Plasmid | PVT15867
Nova Lifetech

pCDNA3-Flag mTOR Plasmid | PVT14613
Nova Lifetech