EV-Entry System for Exosome Delivery (5 rxn)

See how well the EV-Entry System enhances exosome-mediated cargo delivery

The EV-Entry System increases the efficiency of exosome-mediated delivery of proteins to target cells.

In Figure 1, Exo-Green is used to label exosomal proteins, and then EV-Entry enhanced transfer of Exo-Green-labeled exosomal proteins to recipient cells is monitored using fluorescence microscopy. In Figure 2, enhanced transfer of a specific exosomal protein—XPack-Luciferase (Cat.# XPAK732PA-1)—is shown. In both cases, the EV-Entry System increases the amount of cargo delivered.

Figure 1. The EV-Entry System enhances delivery of Exo-Green-labeled exosomal proteins to recipient cells. Exosomes were labeled with Exo-Green and added to HEK293 cells with or without the EV-Entry reagent, and cells imaged after 18 hours. Cells that received exosomes with the EV-Entry reagent showed remarkably enhanced uptake of exosomes and release of exosomal cargo into the cytoplasm as demonstrated by the staining pattern of exosomal proteins in the recipient cells using a very brief exposure time.

Figure 2. The EV-Entry System enhances delivery of XPack-Luciferase to recipient cells. HEK 293 cells were transfected with XPack-Luciferase, exosomes were harvested, and then these XPack-Luciferase-containing exosomes were added to naive HEK 293 cells with or without the EV-Entry reagent. After 18 hours, target cells were washed multiple times with PBS, lysed and luciferase activity measured. Cells that received XPack-Luciferase exosomes treated with the EV-Entry reagent demonstrated over 11-fold greater luciferase activity compared to untreated exosomes, demonstrating that EV-Entry enhances cargo delivery.

The EV-Entry System also increases the efficiency of RNA cargo delivery.

In Figure 3, XMIR Texas Red-labeled Positive Control RNA Oligos (Cat.# XMIR-POS) are used to label exosomes and then EV-Entry enhanced transfer of the XMIR Texas Red-labeled RNA Oligo to recipient cells is monitored using fluorescence microscopy. As with the delivery of protein cargo, the EV-Entry System improves the efficiency of exosome-mediated delivery of RNAs.