pMSCV-Human Foxp3-EF1-GFP-T2A-Puro piggyBac transposon plasmid

Robust overexpression of Foxp3

Figure 2. SBI’s human and mouse Foxp3 lentivector and PiggyBac plasmids provide robust Foxp3 overexpression. The human and mouse Foxp3 lentivectors Cat.# TCL200A-1 (human) and Cat.# TCL100A-1 (mouse) and the PiggyBac transposon constructs Cat.# TCL200PB-1 (human) and Cat.# TCL100PB-1 (mouse) were transiently transfected into 293FT cells. After 24 hours, cellular lysates were prepared and Western blot analyses performed to detect the Foxp3 protein expression using an anti-Foxp3 antibody that detects both human and mouse proteins. Robust overexpression of both human (lanes 1,3) and mouse (lanes 2,4) Foxp3 proteins compared to the non-transfected cell lysate control (lane 5) can be clearly observed, with tubulin as a loading control.

Figure 3. SBI’s MSCV-Human Foxp3-EF1α-GFP-T2A-Puro Jurkat Cell Line shows strong Foxp3 overexpression. Human Jurkat T cells were transduced with either Cat.# TCL200A-1 (human) or Cat.# TCL100A-1 (mouse) pre-packaged lentivirus and stable cell lines established using puromycin selection for seven days. Overexpression of Foxp3 was evaluated by measuring GFP levels using flow cytometry and Western blot analysis of cellular lysates and using anti-Foxp3 antibody. Both methods show that SBI’s Foxp3-overexpressing Jurkat Cell Line possesses strong Foxp3 overexpression.